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Last update: Pátková Vlasta (16.11.2018)
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Last update: Pátková Vlasta (16.11.2018)
Students will be able to: Formulate mass and heat balances of different types of bioreactors. Understand the transport and reaction processes taking place in various types of enzyme and cell bioreactors. Solve mathematical models of bioreactors. Orient in modern DNA and protein technologies. |
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Last update: Přibyl Michal prof. Ing. Ph.D. (04.05.2022)
Blanch H.W., Clark, D.S., Biochemical Engineering, 2nd edition, Marcel Dekker, 1997, 0-8247-0099-6. Harrison R.G., Todd P., Rudge S.R., Petrides D.P., Bioseparations science and engineering, 2nd edition, Oxford University Press, 2015, 978-0-19-539181-7. Villadsen J., Lee S.Y., Nielsen J., Stephanopoulos G. (eds.), Fundamental Bioengineering, Wiley-Blackwell, 2016, 978-3527336746. Alberts B. et al., Essential Cell Biology, 5th edition, Garland Science, 2019, 978-0-393679533. |
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Last update: Pátková Vlasta (16.11.2018)
None. |
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Last update: Pátková Vlasta (16.11.2018)
1. Cell types, eukaryotic and prokaryotic cells, cell structure, chemical composition of cells, from DNA to protein, cell cycle, cell energetics, energetics of microbial growth. 2. Enzymes, enzyme reactions, kinetics of enzyme reactions, inhibition of enzymes, effects of pH, ionic strength, temperature. 3. Immobilized enzymes and microbial cells, immobilization methods, effect of immobilization on kinetics of enzyme reactions. 4. Transport in systems with immobilized biocatalysts, limiting process. 5. Kinetics of microbial growth and metabolite production, models of microbial growth. 6. Cell chemotaxis, mathematical model of chemotaxis in spatially one-dimensional system. 7. Types of microbial interactions, mathematical models of microbial interactions, competition and influence of chemotaxis, predator-prey systems. 8. Bioreactors with enzymes and microbial cells, examples of the use, bioreactor control. 9. Mass and heat transfer in bioreactors, transport of oxygen in fermeters, oxygen solubility, kLa. 10. Thermal sterilization, sterilization equipment, heat transfer coefficients. 11. Separation and purification of microbial products, separation of cells from the culture medium, destruction of cells, product separation and purification. 12. Typical sequences of separation steps, economic principles of the downstream process. 13. DNA and protein technology. DNA sequencing, DNA amplification, cDNA libraries, gene engineering. 14. Recombinant proteins, genetically modified organisms (preparation, benefits and risks, examples), preparation of special proteins in genetically engineered modified organisms, preparation of monoclonal and polyclonal antibodies. |
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Last update: Pátková Vlasta (16.11.2018)
Chemical engineering Biochemistry |
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Last update: Pátková Vlasta (16.11.2018)
In oral exam, a student answers three questions from a list of questions. |