Molecular genetics and DNA analysis - S320081
Title: Molecular genetics and DNA analysis
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
Teaching methods: full-time
Level:  
For type:  
Guarantor: Ruml Tomáš prof. Ing. CSc.
Is interchangeable with: AB320015
Examination dates   Schedule   
Annotation -
Last update: Kubová Petra Ing. (23.05.2016)
The subject is focused on understanding heredity principles especially mechanisms and regulation of transfer of genetic information and gene expression in prokaryotic and eukaryotic organisms. Special emphasis will be put on the main regulatory processes mediated by proteins and RNA regulatory molecules. Other focus is on understanding the variability of genetic information due to mutations and recombination. The subject offers information on the most important methods of DNA analysis as DNA and RNA isolation, their labeling, sequencing and analysis of selected sequences by using polymerase chain reaction and comparison of data with available databases.
Aim of the course -
Last update: Kubová Petra Ing. (23.05.2016)

Students will know:

Basic principles of molecular genetics and the relationship of their structure and function.

Principles of regulation of transfer of genetic information and its variability due to mutations and recombination

Mechanisms involved in mutation repair.

Basic processes of gene expression as regulation of transcription, in prokaryotes and eukaryotes and posttranscriptional modifications of eukaryotic mRNA, regulation of stability of mRNA and translation, role of small RNAs.

Basic principles of work with DNA, methods of DNA analysis and basic applications of polymerase chain reaction

Literature -
Last update: Kubová Petra Ing. (23.05.2016)

R: Lodish, H.; et al. Molecular cell biology, 6th ed.;W.H.Freeman & Co Ltd, 2008.

R: Albrets B; et al. Molecular biology of the cell, 5th ed. Garland Science, 2008.

R: Clark D.P. Molecular biology, Elsevier Inc., 2005.

R: Hitler J.M. Forensic DNA Typing, 2nd ed. Elsevier 2005.

Learning resources -
Last update: Kubová Petra Ing. (23.05.2016)

none
Syllabus -
Last update: Kubová Petra Ing. (23.05.2016)

1. Historic overview and basic genetic laws and terms.

2. Structure and function of nucleic acids, genome organization

3. Replication, DNA polymerases, replisome complex.

4. Mutations and repair mechanisms.

5. Recombination, mitotic and meiotic gene segregation, transposition.

6. Regulation of transcription.

7. Posttranscriptional modifications of RNA.

8. Regulatory role of RNA.

9. Translation, posttranslational modifications.

10. Principles of work with DNA and RNA.

11. Methods of analysis of DNA sequence, genome mapping.

12. Labeling of nucleic acids, application of probes.

13. Polymerase chain reaction and its applications.

14. DNA databases.

Entry requirements -
Last update: Kubová Petra Ing. (23.05.2016)

To enroll in the course it is required enrollment in courses N110004 A N320004

Registration requirements -
Last update: Kubová Petra Ing. (23.05.2016)

Biochemistry I