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Poslední úprava: Kopecká Blanka Ing. Ph.D. (29.08.2018)
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Poslední úprava: Kopecká Blanka Ing. Ph.D. (29.08.2018)
Students will be able to:
Isolate DNA and perform basic manipulations with recombinant DNA. Prepare vectors for genetic modification of various cell types.
Modify and label DNA, apply DNA labeled probes for identification of specific sequences.
Modify genes by directed mutagenesis by directed mutagenesis, extension by sequences for detection and purification by affinity chromatography.
Analyze production of heterologous proteins and isolate them from various cell types.
Analyze protein interactions. Construct gene libraries. |
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Poslední úprava: Ruml Tomáš prof. Ing. CSc. (31.08.2018)
R: Green M. R., Sambrook J., Molecular Cloning: A Laboratory Manual (Fourth Edition); Cold Spring Harbor Laboratory Press 2012 |
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Poslední úprava: Ruml Tomáš prof. Ing. CSc. (26.09.2018)
1. Introduction genetics 1 2. Introduction genetics 2 3. Introduction genetic engineering 4. Restriction endonucleases 5. Sequence analysis 6. Gene expression 1 7. Gene expression 2 biotechnology 8. PCR 9. DNA sequencing 10. Forensic analysis 11. Protein interactions 12. Gene therapy 1 13. Gene therapy 2 14. Interactions assessment
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