Students will be able to:

1. Work with a microscope and prepare samples for microscopy.

2. Basic cultivation techniques, including confirmation and evaluation.

3. Use bioindication to assess the evaluated matrix (sample, environment, technology).

4. Apply the results of matrix analysis in practice.

R:Říhová Ambrožová J.,Baumruková L.,Zuzáková J.,Vejmelková D.,Novotná J.,Biocenózy vodních biotopů – Biocenosis of Water Biotopes – Biocenosis de hábitat acuático,2020,ISBN 978-80-7592-064-5

R:Zelenakova M.,Hlavínek P.,Negm A.M.,Management of Water Quality and Quantity,2019,ISSN: 2364-6934,ISBN: 978-030-18358-5

R:Brown A.,Smith H.,Benson´s Microbiological Applications,Laboratory Manual in General Microbiology, www.mhhe.com,2014,ISBN: 978-0-07-76682-0

A:Říhová Ambrožová J.,Encyklopedie hydrobiologie,Encyclopedia of hydrobiology,el.version 1.0,2007,ISBN 978-80-7080-007-2,(e-learning in english)

A:Říhová Ambrožová J.,Atlas mikroorganismů,Atlas of microorganisms,2014,ISBN 978-80-7414818-7,ISBN 978-80-7414-855-2(online:pdf),photos of microorganisms for microscopy and determination

1. Specification of biological methods (principles, procedures, standards)

2. Methods of sampling, analysis, standards (legislation)

3. Specification of organisms (algae, micromycetes, bacteria, protozoa, multicellular)

4. Microscope, microscopy and techniques (preparations, staining, fluorescence)

5. Methods and principles of work in a microbiological laboratory (dilution, samples, media)

6. Cultivation techniques (aerobic, anaerobic)

7. Usage of microscopy in water technology (water and wastewater treatment etc.)

8. Evaluation of water quality, growths and biota, saprobiology (bioindication)

9. Usage of microbiological methods in the evaluation of water technology (water and wastewater treatment etc.)

10. Bioindicators, indicators of hygienic water safety and water quality

11. Eutrophication, pollution, toxicity

12. Screening methods and microbiotests

None.

70% presence in laboratories, 30% protocols and testes.