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The course focuses on the explanation of processes taking part in photoluminescence and affecting its analytical applications. Overview of basic applications of photoluminescence in analytical, industrial and research practice is given.
Last update: Fähnrich Jan (27.08.2013)
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Students will be able to: Select appropriate photoluminescence method in cases where it is suitable for the solution of a given task Use this method in correct manner acknowledging factors which may deteriorate the reliability of results Last update: Fähnrich Jan (27.08.2013)
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R: Lakowicz J.R., Principles of Fluorescence Spectroscopy, Springer, 2006, ISBN 13: 978-0387-31278-1 Powered by TCPDF (www.tcpdf.org) Last update: VED402 (01.10.2013)
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1. Basic concept: energetic states of molecule, Jablonski diagram 2. Franck-Condon principle, transition moment, quantum yield and quantum efficiency 3. Instrumental equipment: sources of excitation radiation, monochromators, cuvettes 4. Detection of luminescence radiation 5. Measured dependencies: excitation-emission matrix, luminescence quenching, Stern-Volmer equation 6. Measurement of quantum yield using absolute and relative methods 7. Analytical applications of luminescence: qualitative analysis, the effect of substance structure 8. Quantitative analysis 9. Luminescence in organic and inorganic analysis 10. Detection in chromatography, derivatization methods 11. Special techniques: time resolved luminescence, correlation spectrometry 12. Methods based on energy transfer, Shpolski effect 13. Low temperature spectrometry, polarization of luminescence, room temperature phosphorescence 14. Chemiluminescence, kinetic measurements, photoluminescence sensors Last update: Fähnrich Jan (27.08.2013)
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Encyclopedia of Analytical Chemistry Copyright © 1999-2013 by John Wiley and Sons, Inc. Electronic Absorption and Luminescence http://onlinelibrary.wiley.com/book/10.1002/9780470027318/toc Last update: Fähnrich Jan (27.08.2013)
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Analytical chemistry Physics Last update: Fähnrich Jan (27.08.2013)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Účast na přednáškách | 0.5 | 14 | ||
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 0.5 | 14 | ||
Příprava na zkoušku a její absolvování | 1 | 28 | ||
2 / 2 | 56 / 56 |
Coursework assessment | |
Form | Significance |
Oral examination | 100 |