Subjects

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Liqid chromatography in Analysis of Foods and Natural Products - D323016

Title:	Kapalinová chromatografie v analýze potravin a přírodních produktů
Guaranteed by:	Department of Food Analysis and Nutrition (323)
Faculty:	Faculty of Food and Biochemical Technology
Actual:	from 2012 to 2015
Semester:	both
Points:	0
E-Credits:	0
Examination process:
Hours per week, examination:	0/0, other [HT]
Capacity:	winter:unknown / unknown (unknown) summer:unknown / unknown (unknown)
Min. number of students:	unlimited
Language:	Czech
Teaching methods:	full-time
Teaching methods:	full-time
Level:
For type:
Note:	course is intended for doctoral students only you can enroll for the course in winter and in summer semester

Guarantor:	Schulzová Věra doc. Dr. Ing. Tomaniová Monika Ing. Ph.D.

Examination dates Schedule

Annotation -

The course focuses on the principles of separation by liquid chromatography, the column selection, mobile phase, and detection. Special focus is given to the selection and optimization of chromatographic conditions. Development and validation of analytical methods is discussed in detail. In specific cases the various problems and their solutions are discussed. Attention focusses on the use of liquid chromatography in analysis of foods and natural products (monitoring of biologically active substances) and residual analysis.

Last update: Schulzová Věra (03.03.2014)

Aim of the course -

Students will be able to:

Understand the basic principles of liquid chromatography

Optimize the separation and detection conditions

Applied HPLC technique for detection of natural food components and contaminants

Last update: Schulzová Věra (03.03.2014)

Literature -

Books

Food Analysis by HPLC - Nollet (2000)

Practical HPLC - Meyer (1996)

Practical HPLC Method development - L.R.Snyder, J.J. Kirkland, J. L. Glajch (1997)

LC/MS a practical users guide - M. C. McMaster (2005)

Pitfalls and Errors of HPLC in Pictures - V. R. Meyer (2006)

electronic book:

HPLC and CE: Principles and Practice

Andrea Weston and Phyllis R. Brown (1997)

http://www.sciencedirect.com/science/book/9780121366407

Journals:

Journal of Chromatography

Chromatographia

LC-GC International

Journal of Liquid Chromatography

www

LC Troubleshooting: http://www.lcgceurope.com/lcgceurope/article/articleList.jsp?categoryId=535

HPLC guide

http://www.separationsnow.com/coi/cda/home.cda;jsessionid=4EAB9422438DB4C4A206CBBE16FD089A?chId=4

High Performance Liquid Chromatography (HPLC): A Users Guide http://kerouac.pharm.uky.edu/ASRG/HPLC/hplcmytry.html

http://kerouac.pharm.uky.edu/asrg/hplc/applications.html

Practical HPLC Method Development, 2nd Edition (Hardcover)

http://www.amazon.com/exec/obidos/ASIN/047100703X/entertmusicvi-20/103-0437811-9739029

Links to Various Resources, topics, courses

http://www.forumsci.co.il/HPLC/links.html

HPLC Chromatography Books

http://www.chromtech.net.au/amazon-hplc-books1.cfm

Basic Liquid Chromatography

http://hplc.chem.shu.edu/NEW/HPLC_Book/

HPLC portal

http://www.hplcweb.com/

The HPLC troubleshooter

http://www.dq.fct.unl.pt/QOF/hplcts.html

useful HPLC links

http://www.lcresources.com/resources/reslinks.html

e-learning HPLC

http://www.academysavant.com/elearning/elearnHPLC

Last update: Schulzová Věra (28.02.2014)

Learning resources -

http://web.vscht.cz/schulzov

Last update: Schulzová Věra (28.02.2014)

Syllabus -

1 Introduction, principles of separation, main principles

2 New trends

3 Mobile phases

4 Methods development - optimization of the chromatographic separation process

5 Factors affecting the HPLC determination

6 Problems in HPLC and their solutions

7 Detection - conventional detectors

8 Detection - mass detectors

9 Derivatization techniques, injection techniques

10 Application of HPLC in food analysis

11 Application of LC / MS analysis of food

12 Case studies and their presentation

13 Case studies and their presentation

14 The final test, examination

Last update: Schulzová Věra (03.03.2014)

Entry requirements -

Analytical Chemistry

Food Analysis

Last update: Schulzová Věra (28.02.2014)

Course completion requirements -

written examination

project presentation and discussion

Last update: Schulzová Věra (03.03.2014)

Coursework assessment
Form	Significance
Regular attendance	10
Defense of an individual project	30
Examination test	60