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The general part of the course presents advanced topics of enzyme kinetics, enzyme assays with a focus on "high throughput" approaches, regulation of enzymes in the cell, medicinal enzymology, enzyme engineering and mechanisms of enzymatic reactions. The second part will present applied enzymology, namely industrial production of enzymes and current trends in application of enzymes in biotechnology, analytical chemistry, clinical diagnostics and organic synthesis.
Last update: Spiwok Vojtěch (05.03.2014)
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Written and oral exam, written essay. Last update: Spiwok Vojtěch (05.03.2014)
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A:A.G. Marangoni (Ed.): Enzyme Kinetics. John Wiley & Sons, Inc. 2003, ISBN: 9780471159858 (printed), 9780471267294 (online) A:H. Bisswanger: Enzyme Kinetics. Wiley-VCH Verlag GmbH, 2002, ISBN: 9783527303434 (printed), 9783527601752 (online) A:R.J. Whitehurst, M. van Oort: Enzymes in Food Technology, Blackwell Publishing Ltd, 2010, ISBN: 9781405183666 (printed), 9781444309935 (online) A:J. Polaina, A.P. MacCabe: Industrial Enzymes : Structure, Function and Applications, Springer Dordrecht NLD, 2007, ISBN: 9789048173549 (printed), 9781402053771 (online) A:J.R. Whitaker, A.G.J. Voragen, D.W.S. Wong, Handbook of Food Enzymology, CRC Press 2002 ISBN: 9780824706869 (printed), 9780203910450, (online) Last update: Spiwok Vojtěch (05.03.2014)
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1. Enzyme kinetics, Michaelis-Menten kinetics, multi-substrate reactions 2. Enzyme inhibition 3. Enzyme assays, application of optical, separation, electrochemical and other methods 4. Regulation of enzyme activity by non-covalent interactions and covalent modifications, methods of enzyme research 5. Enzymes as drug targets, drug design 6. Explanation of enzyme catalysis, mechanisms of enzymatic reactions, methods of mechanism studies 7. Enzyme engineering, rational and directed evolution 8. Enzyme sources, searching and production of new enzymes 9. Application of enzymes in food and non-food technologies 11. Enzymes as analytical sensors and diagnostic tools 12. Biotransformations, enzymes in organic synthesis Last update: Spiwok Vojtěch (05.03.2014)
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http://web.vscht.cz/spiwokv/enzymologie/ http://biomikro.vscht.cz/vyuka/?Predmet=ae Last update: Spiwok Vojtěch (05.03.2014)
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Students will be able to:
Design and apply advanced methods for characterisation and application of enzymes in biochemistry research, drug design, food and non-food technologies and analytical chemistry
Last update: Spiwok Vojtěch (05.03.2014)
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None. Last update: Spiwok Vojtěch (05.03.2014)
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| Coursework assessment | |
| Form | Significance |
| Regular attendance | 20 |
| Examination test | 40 |
| Oral examination | 40 |