SubjectsSubjects(version: 965)
Course, academic year 2019/2020
  
Isolation and Separation Methods - D323011
Title: Izolační a separační metody
Guaranteed by: Department of Food Analysis and Nutrition (323)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2014 to 2022
Semester: both
Points: 0
E-Credits: 0
Examination process:
Hours per week, examination: 0/0, other [HT]
Capacity: winter:unknown / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Level:  
Note: course is intended for doctoral students only
can be fulfilled in the future
you can enroll for the course in winter and in summer semester
Guarantor: Poustka Jan prof. Dr. Ing.
Pulkrabová Jana prof. Ing. Ph.D.
Examination dates   Schedule   
This subject contains the following additional online materials
Annotation -
The course is focused on the principles and advanced applications of modern isolation and separation techniques including representative detection methods. Methods are discussed with regard to practical aspects and problems that are normally encountered. Framework of studied methods: isolation in gaseous, liquid, supercritical and solid phase; gas and liquid chromatography, including conventional detectors, mass spectrometry.
Last update: Poustka Jan (03.03.2014)
Course completion requirements -

The successful completion of a written test or alternatively an oral examination.

Last update: Poustka Jan (03.03.2014)
Literature -

Churáček, J. a kol.: Analytická separace látek, SNTL, Praha, ISBN 80-03-00569-8, 1990.

Pawliszyn J.: Solid phase microextraction: Theory and Practice. Wiley-VCH, Inc. NY, USA, ISBN 0-471-19034-9, 1997.

Dean, J.R.: Extraction Methods for Environmental Analysis, J.Wiley&Sons, Ltd., Chichester, Anglie, ISBN 0-471-98287-3, 1998.

Grob, R.L. (Editor): Modern Practice of Gas Chromatography, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-59700-7, 1995.

Willoughby, R., Sheehan, E., Mitrovich, S.: A Global View of LC/MS: How to Solve Your Most Challenging Analytical Problems, Global View Publishing, Pittsburg, Pennsylvania, USA, ISBN 0-9660813-0-7, 1998.

Snyder,L.R., Kirkland, J.J., Glajch, J.L.: Practical HPLC Method Development, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-00703-X, 1997.

Rood, D.: A Practical Guide to the Care Maintenance and Troubleshooting of Capillary Gas Chromatographic Systems, Hüthig GmbH, Heidelberg, Německo, ISBN 3-7785-2319-8, 1995.

Scientific papers.

Last update: Poustka Jan (13.09.2023)
Syllabus -

1. General characteristics of analytical procedure and classification of techniques

2. Purge & trap, head space, supercritical fluid extraction

3. Liquid-liquid extraction, liquid micro-extraction

4. Solid-liquid extraction, various means of implementation (ASE-PLE, MASE)

5. Solid phase extraction

6. Solid phase micro-extraction

7. Adsorption chromatography, dispersive solid phase extraction (MSPD, QuEChERS)

8. Gel permeation chromatography

9. Gas chromatography - factors influencing separation, injection techniques

10. Gas chromatography - columns, detectors

11. Gas chromatography - large volume injection; GCxGC

12. Liquid chromatography - factors influencing separation

13. Liquid chromatography - columns, detectors

14. Mass spectrometry

Last update: Poustka Jan (03.03.2014)
Learning resources -

https://e-learning.vscht.cz/

Last update: Poustka Jan (13.09.2023)
Learning outcomes -

Students will be able to understand:

1. Principles, technical configurations and application potential of sample preparation methods

2. Principles, technical configurations and application potential of gas and liquid chromatography including the application of conventional detectors

3. Principles, technical configurations and application potential of mass spectrometric methods based on various principles of ion separation

Last update: Poustka Jan (03.03.2014)
Registration requirements -

No requirements

Last update: Poustka Jan (03.03.2014)
Coursework assessment
Form Significance
Examination test 100

 
VŠCHT Praha