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The course provides students with theoretical fundamentals of immunochemical methods and an overview of the most commonly used procedures in practice, followed by hands-on training in laboratory techniques used in various types of immunochemical methods.
Last update: Holubová Barbora (19.01.2026)
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Oral presentation of results and completed written exams (student must obtain at least 50%). Last update: Holubová Barbora (19.01.2026)
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Daussant J., Desvaux F.-X., Introduction to immunochemical techniques for medical diagnosis, food quality control and environmental testing, 2007, ICT Press, 9788070806418 (z) Fukal L., Holubová B.: Imunochemie a imunoanalýza. Praha 2007, ISBN 978-80-239-8903-8 (d) Last update: Holubová Barbora (15.09.2023)
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1. Antigen and antibodies 2. Overview of immunochemical methods 3. ELISA 4. Immunochromatographic test (ICT) 5. Immunohistochemistry and immunocytochemistry 6. Laboratory tasks: ICT, ELISA, immunoblot Last update: Holubová Barbora (19.01.2026)
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Lectures and manuals in electronic form published on e-learning.vscht.cz. Last update: Holubová Barbora (15.09.2023)
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Upon completing the course, the student will be able to explain the principles of antigen–antibody interactions and describe the major immunochemical methods (ELISA, immunochromatographic test, immunoblot, and immunohistochemistry). The student will be able to independently perform basic immunochemical techniques and correctly process and interpret the obtained data. They will be able to identify possible sources of measurement errors and propose ways to minimize them. Furthermore, the student will be able to present the results of their individual and team laboratory work. Last update: Holubová Barbora (16.03.2026)
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Entry requirements within the scope of the Biochemistry I course curriculum. Last update: Holubová Barbora (19.01.2026)
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| Teaching methods | ||||
| Activity | Credits | Hours | ||
| Účast v laboratořích (na exkurzi nebo praxi) | 2 | 56 | ||
| Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 0.5 | 14 | ||
| Příprava na zkoušku a její absolvování | 0.5 | 14 | ||
| 3 / 3 | 84 / 84 | |||
| Coursework assessment | |
| Form | Significance |
| Regular attendance | 50 |
| Report from individual projects | 25 |
| Examination test | 25 |
