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In lectures, students learn about modern methods of trace element analysis based on the principles of atomic spectroscopy. Introductory part of the course is devoted to theoretical principles, the structure of the atom, energy transitions in the electron shell of atoms and basic structural elements used in atomic spectroscopy. The main part of the course comprises atomic absorption spectroscopy, which is currently the most widely used technique of elemental analysis. Students learn the used radiation sources, methods of atomization interference correction methods and some special approaches to the analysis (generation of volatile compounds, electrolytic deposition of analyte). Another series of lectures is devoted to emission spectral analysis, students will learn various methods of excitation spectra, such as spark, arc, glow discharge, plasma and laser. Attention is also paid to the atomic fluorescence spectroscopy, the X-ray, electron and mass spectroscopy. In the lectures on applications, students get acquainted with work in the trace lab, use of coupled techniques for speciation analysis, the methods of preparation of various types of samples (water, biological materials, ores and rocks) and the possibilities of using atomic spectroscopy techniques for in situ analysis.
In the laboratory, students learn the practical application of some of these techniques (different types of atomic absorption spectroscopy, mass spectroscopy, inductively coupled plasma and X-ray fluorescence spectroscopy) for elemental analysis of major and trace amounts of elemets in rocks. Samples collected in areas Inter-university underground laboratory Josef Will be analyzed.
Last update: MESTEKO (15.09.2013)
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R: Cullen M.: Atomic Spectroscopy in Elemental Analysis, Blackwell, ISBN 1-84127-333-3 R: José A.C. Broekaert: Analytical Atomic Spectrometry with Flames and Plasmas, Wiley, ISBN 978-3-527-31282-5 Skripta (in Czech): Černohorský T., Jandera P.: Atomová spektroskopie, Univerzita Pardubice 1997. ISBN 80-7194-114-X R: Ebdon L., Evans E.H., Fisher A.S., Hill S.J.: An Introduction to Analytical Atomic Spectrometry. Willey 1998, ISBN 978-0-471-97418-5 Last update: MESTEKO (20.02.2018)
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Passing labs and acknowledgement of protocols by assistant professor. An oral examination. Student can use only two reparative terms. Last update: MESTEKO (15.09.2013)
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1. Structure of atoms.
2. Interaction of electrons and electromagnetic radiation, width and intensity of spectral lines.
3. Fundamentals of optics, construction of monochromators and polychromators, construction of radiation detectors.
4. Atomic absorption spectrometry, basic instrumentation - radiation sources, atomizers.
5. Atomic absorption spectrometry: interference and background correction, signal optimization and processing.
6. Atomic absorption spectrometry: special techniques - hydride generation, mercury determination, electrodeposition.
7. Atomic fluorescence spectrometry.
8. Flame emission spectrometry, spectrometry with electric arc and spark excitation, rotating electrode spectroscopy, spectroscopy with glow discharge.
9. Inductively coupled and DC plasma optical emission spectrometry, LIBS
10. Inductively coupled plasma mass spectrometry: basic instrumentation - ion optics, ion filters and detectors.
11. Inductively coupled plasma mass spectrometry: interference and methods of their elimination; special techniques - laser ablation, analysis of nanoparticles, connection with electrothermal evaporation.
12. Hyphenated techniques in speciation analysis of trace elements.
13. X-ray fluorescence spectrometry.
14. Preparation of samples for elemental trace analysis, principles of work in a trace laboratory.
Last update: MESTEKO (10.04.2018)
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http://web.vscht.cz/~koplikr/Atomov%c3%a1%20%20spektrometrie.pdf Last update: MESTEKO (03.09.2013)
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Students completing the course will acquire theoretical and practical knowledge of the methods of elemental analysis using optical spectroscopy (absorption, emission and fluorescence), X-ray spectrometry and mass spectrometry. They will be familiar with the various methods of sample preparation and problems of cleanness in trace laboratory. Last update: MESTEKO (15.09.2013)
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Basic knowledge of analytical chemistry Last update: MESTEKO (03.09.2013)
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| Teaching methods | ||||
| Activity | Credits | Hours | ||
| Konzultace s vyučujícími | 0.5 | 14 | ||
| Účast v laboratořích (na exkurzi nebo praxi) | 1 | 28 | ||
| Účast na přednáškách | 1 | 28 | ||
| Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 1 | 28 | ||
| Příprava na zkoušku a její absolvování | 1.5 | 42 | ||
| 5 / 5 | 140 / 140 | |||
| Coursework assessment | |
| Form | Significance |
| Report from individual projects | 50 |
| Oral examination | 50 |