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The course is focused on principles of liquid chromatography compound separation, especially for column and mobile phase selection, and the strategy of choosing a suitable detection. Attention is paid to the setting and optimization of chromatographic conditions, the development and validation of liquid chromatography analytical methods is discussed in detail. The individual issues that the user may encounter and their solutions are discussed using specific examples. Attention is focused on the use of liquid chromatography in food and natural product analysis monitoring of nutritionally important and biologically active compounds, the specifics of residual analysis are presented.
Last update: Schulzová Věra (24.05.2018)
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written examination oral exam project presentation and discussion Last update: Schulzová Věra (14.06.2018)
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R: Otles S. (ed.): Handbook of Food Analysis Instruments. CRC Press, Boca Raton, USA, 2009, e-zdroje VŠCHT; ISBN 9781420045666 R: LC-GC, http://www.chromatographyonline.com A: Wrolstad R.E. et al. Handbook of Food Analytical Chemistry, Wiley, 2005, e-zdroj VŠCHT; ISBN 9780471663782 A: Food Analytical Methods, elektronický časopis, Springer, e-zdroj VŠCHT; ISSN 1936-976X A: Troubleshooting HPLC systems, A bench manual, Sadek PC, Wiley, 2000, ISBN 0-471-17834-9 A: Food Analysis by HPLC, Second Edition, Leo M.L. Nollet, CRC Press, 2000, ISBN 082478460X, 9780824784607 A: Practical HPLC Method Development, Lloyd R. Snyder, Wiley, 2011, ISBN 0-471-00703-X A: LC/MS a practical users guide, M. C. McMaster, Wiley, 2005, ISBN 13987-0-471-65531-2 A: Pitfalls and Errors of HPLC in Pictures, Third Edition, Veronika R. Meyer, 2006, ISBN 3-527-31377-X, https://download.e-bookshelf.de/download/0003/7214/07/L-G-0003721407-0002367262.pdf A: Journal of Chromatography, ISSN: 0021-9673, https://www.journals.elsevier.com/journal-of-chromatography-a/ A: Basic Liquid Chromatography, http://hplc.chem.shu.edu/NEW/HPLC_Book/ A: HPLC portal, http://www.hplcweb.com/ A: Useful chromatography links - LC Resources, http://www.lcresources.com/resources/reslinks.html A: Introduction to HPLC, Dr. John Dolan and Dr. Dennis Saunders, e-Learning programs, http://www.academysavant.com/elearning/elearnHPLC.htm Last update: Schulzová Věra (28.05.2018)
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Teaching lecturers, student presentations - case studies Last update: Schulzová Věra (19.07.2018)
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None Last update: Vlčková Martina (19.07.2018)
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1. Principles of liquid chromatography compound separation, basic relations 2. Analytical columns selection and properties 3. Mobile phases selection and their properties 4. A strategy for detector selecting 5. Setting and optimization of chromatographic conditions 6. Development and validation of analytical methods using liquid chromatography 7. Troubleshooting and common problems in liquid chromatography 8. The use of liquid chromatography in food and natural product analysis monitoring of nutritionally important and biologically active compounds 9. Specifics of residual analysis 10. Troubleshooting: specific cases and methods of solving problems in liquid chromatography 11. Presentation of case studies, discussion and evaluation Last update: Schulzová Věra (24.05.2018)
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http://web.vscht.cz/schulzov Last update: Pátková Vlasta (18.05.2018)
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Students will be able to:
Last update: Pátková Vlasta (18.05.2018)
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they are not Last update: Schulzová Věra (14.06.2018)
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None Last update: Vlčková Martina (19.07.2018)
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| Coursework assessment | |
| Form | Significance |
| Regular attendance | 5 |
| Defense of an individual project | 30 |
| Examination test | 50 |
| Oral examination | 15 |