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The course of Bioorganic chemistry covers the exciting and modern interdisciplinary area at the interface between organic chemistry and biochemistry or biology. It combines and compares molecular principles of structures of functions of biomolecules, molecular principles of biological and biochemical processes (organic chemistry of biochemical pathways), chemical syntheses and biosyntheses of biomolecules and biomacromolecules, use of biochemical and biological means in organic synthesis (biocatalysis), use of chemical means in studying and modulation of biological processes (chemical biology), construction of artificial bioanalogous systems (synthetic biology) and also gives basic physical and chemical principles of modern techniques of biochemistry and molecular biology. It gives the necessary background for an organic chemist to be able to actively collaborate with biochemists or biologists.
Last update: Kubová Petra (14.10.2019)
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Students will be able to: understand the structure and function of biomolecules know molecular principles and reaction mechanisms of biological and biochemical processes chemical syntřheses of biomolecules and bioconjugates applications of biocatalysis in chemistry Last update: Kubová Petra (14.10.2019)
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The course is concluded with a written exam. In case of result 50-70%, an oral part of the exam may be applied. Last update: Kubová Petra (14.10.2019)
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A: Van Vranken D., Weiss G. A.: Introduction to Bioorganic Chemistry and Chemical Biology, Taylor & Francis Group, 2012. 9780815342144 Last update: Kubová Petra (14.10.2019)
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1.Introduction: basic principles of molecular interactions in chemistry and biology, short introduction to cellular biology. 2.Structure and functions of nucleic acids: structures of DNA and RNA, genetic information, other functions of RNA, triplexes and quadruplexes, complexes of nucleic acids with proteins, binding of small molecules, DNA damage. 3.Biosynthesis and metabolism of nucleic acids and their components: biosynthesis of DNA (replication) and RNA (transcription) and their further modifications, catabolism of nucleic acids, biosynthesis and catabolism of nucleobases, nucleosides and nucleotides. 4.Chemical and enzymatic syntheses of nucleic acids and their components: chemical synthesis and modifications of nucleobases, nucleosides and nucleotides, chemical synthesis of oligonucleotides on solid-support, biocatalytical synthesis and construction of oligonucleotides and nucleic acids (primer extension, PCR). 5.Structure and functions of proteins: structures of proteins (primary to quaternary), conformations, conjugates (glyco-, phospho-, metalloproteins etc.), isolation and analysis of proteins, structure and function of peptides 6.Biosynthesis and metabolism of proteins: biosyntheses and metabolism of amino acids, biosynthesis of proteins (translation), posttranslation modifications, cleavage and catabolism of proteins, protein engineering, regulation of gene expression 7.Chemical and biotechnological syntheses of peptides and proteins: chemical syntheses of peptides in solution and on solid support, protecting and activating groups, combination of chemical and enzymatic approaches, recombinant method, peptidomimetics, combinatorial approaches 8.Enzymes and mechanisms of enzymatic reactions: enzymes, cofactors, mechanisms and regulation of enzymatic reactions, thermodynamics and kinetics of enzymatic reactions, inhibition 9.Biocatalytical and biotechnological methods in organic synthesis: use of enzymes, antibodies and whole microorganisms and cell cultures in organic synthesis, enzyme engineering and biotechnology 10.Carbohydrates: structure and functions of carbohydrates and oligosaccharides, metabolism and basic principles in chemical syntheses of oligosaccharides and glycosides, saccharide code, synthetic vaccines 11.Membranes and regulation processes: structures and functions of biomembranes, transports of ions and molecules through membranes, regulation processes - hormones, receptors and signal transduction cascades 12.Biosynthesis and metabolism of other classes of natural compounds: metabolism of lipids, terpenoids, alkaloids, polyketides etc., biomimetic total syntheses of natural compounds 13.Chemical biology: use of chemical means in studying and modulation of biological processes, structural analogues of biomolecules (antimetabolites), chemical genetics, construction of artificial bioanalogous systems, principles of in vitro selection 14.Basic principles of modern methods of biochemistry and molecular biology: chemical principles and insight into production, isolation and analysis of nucleic acids and proteins, sequencing, blotting, PCR etc. Last update: Kubová Petra (01.11.2019)
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Available from the lecturer (copies of all presentations on CD) Last update: Kubová Petra (14.10.2019)
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No. Last update: Kubová Petra (14.10.2019)
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