Subjects

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Immunochemistry: Laboratory - N320026

Title:	Laboratoř imunochemie
Guaranteed by:	Department of Biochemistry and Microbiology (320)
Faculty:	Faculty of Food and Biochemical Technology
Actual:	from 2021
Semester:	summer
Points:	summer s.:3
E-Credits:	summer s.:3
Examination process:	summer s.:
Hours per week, examination:	summer s.:0/4, MC [HT]
Capacity:	unknown / unknown (unknown)
Min. number of students:	unlimited
State of the course:	cancelled
Language:	Czech
Teaching methods:	full-time
Teaching methods:	full-time
Level:

Guarantor:	Hochel Igor doc. Ing. CSc.
Class:	Základní laboratoře
Is interchangeable with:	M320004

Examination dates Schedule

Annotation -

The course is focused on i) acquiring of knowledge and skills in the discipline, ii) detailed description of antigen, hapten and all significant isotypes of antibodies, iii) utilization of antibodies for isolation and preparation of pure preparations, iv) utilization of antibodies in food and clinical testing and environmental monitoring, v) mastering the procedures of various immunochemical assays.

Last update: Hochel Igor (11.07.2013)

Aim of the course -

Students will be able to:

Design the working procedures and to apply immunochmical methods in food and clinical control and in environmental testing.

Last update: Hochel Igor (09.07.2013)

Literature -

R: Daussant J., Desvaux F.-X., Introduction to immunochemical techniques for medical diagnosis, food quality control and environmental testing, 2007, ICT Press, 9788070806418

Last update: Hochel Igor (09.07.2013)

Syllabus -

1. Immunoprecipitation in liquid phase

2. Double diffusion according to Ouchterlony

3. Radial immunodiffusion according to podle Mancini

4. Electrosyneresis

5. Immunoelectrodiffusion according to Laurell

6. Indirect competitive ELISA

7. Non-competitive ELISA

8. GLISA Rapid test

9. SDS-PAGE of proteins, blotting and immunochemical detection of analytes

10. Immunoagglutination

Last update: Hochel Igor (09.07.2013)

Learning resources -

Other study aids:

protocols: http://biomikro.vscht.cz/vyuka/?Predmet=fm

Last update: Hochel Igor (11.07.2013)

Registration requirements -

Biochemistry, Microbiology

Last update: Hochel Igor (09.07.2013)

Teaching methods
	Activity	Credits	Hours
	Účast v laboratořích (na exkurzi nebo praxi)	2	56
	Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi	0.5	14
	Příprava na zkoušku a její absolvování	0.5	14
		3 / 3	84 / 84

Coursework assessment
Form	Significance
Regular attendance	50
Report from individual projects	25
Examination test	25