Subjects

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Isolation and Separation Methods - AM323010

Title:	Isolation and Separation Methods
Guaranteed by:	Department of Food Analysis and Nutrition (323)
Faculty:	Faculty of Food and Biochemical Technology
Actual:	from 2023 to 2026
Semester:	winter
Points:	winter s.:4
E-Credits:	winter s.:4
Examination process:	winter s.:
Hours per week, examination:	winter s.:3/0, Ex [HT]
Capacity:	unlimited / unknown (unknown)
Min. number of students:	unlimited
State of the course:	taught
Language:	English
Teaching methods:	full-time
Level:
Additional information:	https://e-learning.vscht.cz/
Note:	course can be enrolled in outside the study plan enabled for web enrollment

Guarantor:	Poustka Jan prof. Dr. Ing. Pulkrabová Jana prof. Ing. Ph.D.
Interchangeability :	M323010, N323023, S323023
Is interchangeable with:	M323010

Examination dates

This subject contains the following additional online materials

E-learning course

Annotation -

The course is focused on the principles and advanced applications of modern isolation and separation techniques including representative detection methods. Methods are discussed with regard to practical aspects and problems that are normally encountered. Framework of studied methods: isolation methods in gaseous, liquid, supercritical and solid phase; gas and liquid chromatography, including conventional detectors; mass spectrometry.

Last update: Pulkrabová Jana (04.03.2026)

Course completion requirements -

Successful completion of a written examination.

Last update: Pulkrabová Jana (06.03.2026)

Literature -

Recommended:

Pawliszyn, Janusz. Solid Phase Microextraction, Theory and Practice - SPME. New York: Wiley-VCH, 1997, https://vufind.techlib.cz/Record/000963602 s. ISBN 0-471-19034-9.

Dean, J.R.. Extraction Methods for Environmental Analysis. Chichester, UK: J.Wiley&Sons, Ltd., 1998, s. ISBN 0-471-98287-3.

Grob, Robert Lee.. Modern practice of gas chromatography. New York: Wiley, 1995, https://vufind.techlib.cz/Record/000963598 s. ISBN 0-471-59700-7.

Willoughby, R., Sheehan, E., Mitrovich, S.. A Global View of LC/MS: How to Solve Your Most Challenging Analytical Problems. Pittsburg, Pennsylvania, USA: Global View Publishing, 1998, s. ISBN 0-9660813-0-7.

Snyder, Lloyd R., Kirkland, Joseph J., Glajch, Joseph L.. Practical HPLC method development. New York: Wiley, 1997, https://vufind.techlib.cz/Record/000018913 s. ISBN 0-471-00703-X.

Last update: prepocet_literatura.php (19.12.2024)

Requirements to the exam -

Within the extent of the syllabus.

Last update: Pulkrabová Jana (04.03.2026)

Syllabus -

1. General characteristics of analytical procedure and classification of techniques

2. Purge & trap, head space, supercritical fluid extraction

3. Liquid-liquid extraction, liquid micro-extraction

4. Solid-liquid extraction, various means of implementation (ASE-PLE, MASE)

5. Solid phase extraction

6. Solid phase micro-extraction

7. Adsorption chromatography, dispersive solid phase extraction (MSPD, QuEChERS)

8. Gel permeation chromatography

9. Gas chromatography - factors influencing separation, injection techniques

10. Gas chromatography - columns, detectors

11. Gas chromatography - large volume injection; GCxGC

12. Liquid chromatography - factors influencing separation

13. Liquid chromatography - columns, detectors

14. Mass spectrometry

Last update: Pulkrabová Jana (04.03.2026)

Learning resources -

https://e-learning.vscht.cz/

Last update: Poustka Jan (13.09.2023)

Learning outcomes -

Students will be able to understand:

1. Principles, technical configurations and application potential of sample preparation methods

2. Principles, technical configurations and application potential of gas and liquid chromatography including the application of conventional detectors

3. Principles, technical configurations and application potential of mass spectrometric methods based on various principles of ion separation

Last update: Kubová Petra (30.01.2018)

Registration requirements -

No requirements

Last update: Pulkrabová Jana (04.03.2026)

Teaching methods
	Activity	Credits	Hours
	Konzultace s vyučujícími	0.5	14
	Účast na přednáškách	1.5	42
	Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi	0.5	14
	Příprava na zkoušku a její absolvování	1.5	42
		4 / 4	112 / 112