SubjectsSubjects(version: 982)
Course, academic year 2026/2027
  
   
Practical Applications of Microorganisms - B319005
Title: Praktické aplikace mikroorganismů
Guaranteed by: Department of Biotechnology (319)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2026
Semester: summer
Points: summer s.:3
E-Credits: summer s.:3
Examination process: summer s.:
Hours per week, examination: summer s.:2/0, Ex [HT]
Capacity: unknown / unknown (unknown)Schedule is not published yet, this information might be misleading.
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Level:  
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Jarošová Irena prof. Ing. Ph.D.
Pre-requisite : B320007
Examination dates   Schedule   
This subject contains the following additional online materials
Annotation -
Microorganisms are found in all ecosystems, they represent the largest proportion of biomass on Earth. They have the properties, for which they are used in biotechnological processes. Knowledge of the cellular architecture and physiology and also all factors affecting their growth and reproduction is essential for their application. The object of the course is to introduce the possibility resulting in fixing (strengthening) of the used (biosynthesis, biodegradation, biotransformation) characteristics of the microorganism.
Last update: Halecký Martin (11.04.2025)
Course completion requirements -

Attendance at lectures is encouraged but not monitored.

Consultation is available to students on request. An integral part of the course is an e-learning course divided into blocks, within which students are provided with learning support, including electronic textbooks, presentations, lectures and other supplementary materials.

Last update: Jarošová Irena (16.05.2025)
Literature -

Obligatory:

  • Stricoff, R.Scott, Walters, Douglas B.. Laboratory health and safety handbook, A guide for the preparation of a chemical hygiene plan. New York: Wiley, 1990, https://vufind.techlib.cz/Record/000900415 s. ISBN 0-471-61756-3.
  • Forsythe, Hayes. Food Hygiene, Microbiology and HACCP. 1998: AN Aspen Publication, 1998, s. ISBN 0-8342-1815-1.
  • Rochelle, Paul A.. Environmental molecular microbiology, protocols and applications. Wymondham: Horizon Scientific Press, 2001, 264 s. s. ISBN 1-898486-29-8.
  • Madigan, Michael T, Bender, Kelly S, Buckley, Daniel H, Sattley, W. Matthew, Stahl, David A. Brock Biology of Microorganisms, Global Edition. : , , 1 online zdroj (1064 stran) s. ISBN 9781292235103.
  • Stetzenbach, Linda D., Yates, Marylynn V.. The dictionary of environmental microbiology. Amsterdam: Academic Press, 2003, 178 s. s. ISBN 0-12-668000-0.

Last update: Jarošová Irena (31.03.2025)
Teaching methods -

Students are introduced to new material through lectures. Students are also encouraged to discuss topics and actively ask questions. Real examples of the behaviour of microorganisms in different environments are presented, as well as the reasons for such behaviour and the possibilities of adaptation according to the requirements of the type of product - metabolic product, cell biomass...

An expert from a biotechnology company will be invited to some of the lectures to ensure a better link with practice.

Last update: Jarošová Irena (31.03.2025)
Requirements to the exam -

Oral exam

The student will be given 5-7 questions on the topic to answer. Some questions are simply explanations of concepts, others are designed to require more verbal engagement from the student. The exam takes 15-20 minutes to complete. The student registers directly for the exam on the dates and at the times listed.

Last update: Jarošová Irena (16.05.2025)
Syllabus -

1. Safety and organisation of work in different types of microbiological laboratories; requirements for laboratory equipment for work with microorganisms of different groups.

2. Good laboratory practice, accreditation procedure of microbiological laboratories, rules for sampling for microbiological determinations. Methods of detection and identification of pathogenic and indicator

organisms. Classical cultivation methods and rapid detection systems.

3. Disinfectants in biotechnology; determination of surface hygiene, determination of airborne microorganisms. Contamination control - sterilisation, freezing, irradiation and other techniques.

4. Rules for working with genetically modified organisms. Differences between the production of food, food supplements and drugs, production of new types of food.

5. Legislation of the EU and other countries in the food and pharmaceutical industries. Implementation and observance of the rules of the HACCP system, risk analysis. Basic structural types of living systems

6. Cellular nutrition and its relation to cellular functions. Types of cellular reactions

7. Cellular transport mechanisms

8. Physiology of cell growth and cell populations

9. Cell differentiation

10. Cell and external environment, part 1

11. Cell and external environment, part 2.

12. Cell regulation, basic regulatory mechanisms.

13. Regulation of metabolic processes 14. Purposeful Behaviour of the Prokaryotic Cell

Last update: Halecký Martin (11.04.2025)
Learning resources -

https://e-learning.vscht.cz/

Last update: Jarošová Irena (28.03.2025)
Learning outcomes -

Students will be able to: Participate in the preparation of a technological process with regard to the physiological properties of the microorganism used. They will acquire knowledge that will enable them to work in both traditional and emerging areas of biotechnology. They will also be able to apply their knowledge of cell physiology in a subsequent Master's programme. They will be able to find appropriate information on management systems for the quality and safety of food and pharmaceutical products.

Last update: Jarošová Irena (28.03.2025)
Registration requirements -

Microbiology

Last update: Jarošová Irena (28.03.2025)
Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Práce na individuálním projektu 0.4 11
Příprava na zkoušku a její absolvování 1.6 45
3 / 3 84 / 84
Coursework assessment
Form Significance
Oral examination 100

 
VŠCHT Praha