Subjects

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Microscopy and microanalysis in forensic science - B320013

Title:	Mikroskopie a mikroanalýza ve forenzních vědách
Guaranteed by:	Department of Biochemistry and Microbiology (320)
Faculty:	Faculty of Food and Biochemical Technology
Actual:	from 2026
Semester:	both
Points:	3
E-Credits:	3
Examination process:
Hours per week, examination:	2/0, Ex [HT]
Capacity:	winter:unknown / unknown (unknown) summer:unknown / unknown (unknown)
Min. number of students:	unlimited
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Level:
Note:	you can enroll for the course in winter and in summer semester

Guarantor:	Lipov Jan doc. Ing. Ph.D.
Classification:	Chemistry > Analytical Chemistry
Interchangeability :	N320078

Examination dates Schedule

This subject contains the following additional online materials

E-learning course

Annotation -

Microscopy and microanalysis in forensic science should introduce students to extremely large area of use of microscopy and microanalysis in forensic science. As the subject of the forensic investigation could be nearly anything, it is important first to show the basics of microscopic techniques as well as selected spectroscopic techniques used in biological as well as in material science to students. Students will learn the basics of light and electron microscopy first, then the most important areas of forensic investigation are discussed.

Last update: Lipov Jan (23.01.2018)

Course completion requirements -

At least 50% from the written part of the exam.

Passing the oral part of the exam.

Last update: Lipov Jan (25.01.2018)

Literature -

Obligatory:

Murphy, Douglas B., Davidson, Michael W.. Fundamentals of light microscopy and electronic imaging. Hoboken: Wiley-Blackwell, 2013, s. ISBN 978-0-471-69214-0.

Last update: Lipovová Petra (12.06.2025)

Teaching methods -

Lectures - students participate in the teacher's explanation.

Discussion - during the lesson, there is a discussion of interesting questions, space for resolving ambiguities.

Interactive - voluntary participation in anonymous interactive tests on the material being discussed directly in the lesson (possibility of feedback).

Last update: Lipov Jan (26.01.2026)

Requirements to the exam -

The exam consists of a written part, where the student must score at least 50 points out of 100, followed by an oral part of the exam.

Last update: Lipov Jan (26.01.2026)

Syllabus -

1. Introduction to microscopy

2. Optical microscopy - transmitted light

3. Optical microscopy - reflected light

4. Fluorescent microscopy

5. TEM - transmission electron microscopy

6. SEM - scanning electron microscopy

7. X-ray analysis - EDS/WDS, microanalysis

8. Analysis of gun shot residues

9. Analysis of mineral phasis, soil analysis

10. Analysis of glass and post blast residues

11. Analysis of fibers and biological objects

12. Analysis of pigments

13. Ion beam microscopy, analysis of unknown samples

14. Strategy of analysis of unknown sample, picture analysis

Last update: Kubová Petra (23.02.2018)

Learning resources -

http://www.microscopyu.com

http://www.olympusmicro.com

http://zeiss-campus.magnet.fsu.edu

Last update: Kubová Petra (18.12.2017)

Learning outcomes -

Students will be able to:

understand the basics of geometrical optics

understand the basics of light microscopy

orientate themselves in main electron microscopy techniques

properly select the type of investigation technique according to the sample

Last update: Kubová Petra (18.12.2017)

Registration requirements -

none

Last update: Kubová Petra (18.12.2017)

Teaching methods
	Activity	Credits	Hours
	Účast na přednáškách	1	28
	Příprava na zkoušku a její absolvování	2	56
		3 / 3	84 / 84