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The course aims to develop a comprehensive understanding of the design and preparation of a biotechnological experiment, its execution, data analysis, and interpretation of the relationship between experimental conditions and the resulting data. Students will gain the skills necessary to independently carry out basic biotechnological techniques, conduct subsequent analyses, and competently design the entire process, including the final interpretation of findings
Last update: Branská Barbora (25.04.2025)
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Participation in practical lessons Elaboration of reports from individual tasks Last update: Křížová Ivana (21.07.2026)
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Obligatory:
Last update: Branská Barbora (25.04.2025)
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Students work individually or in groups in the laboratory under the supervision of an expert instructor, carrying out assigned tasks. For specific assignments, they collect original data, which they then independently process and present in the form of reports. Last update: Křížová Ivana (21.07.2026)
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Attendance at laboratory sessions is mandatory (each laboratory block begins with an entry quiz to assess students’ preparedness). Successful course completion also requires submission of lab reports for the completed assignments. Last update: Křížová Ivana (21.07.2026)
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1. Introduction to Laboratory Safety, Data Management, and Laboratory Documentation 2. Preparation of Culture Media and Maintenance of Production Microbial Strains 3. Methods for Assessing Cell Growth 4. Molecular Biology Techniques and Their Applications in Biotechnology 5. Production, Isolation, and Analysis of Recombinant Proteins 6. Applications of Fluorescence Microscopy and Flow Cytometry for the Assessment of Microbial Viability
Last update: Křížová Ivana (21.07.2026)
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Upon successful completion of the course, students will be able to perform laboratory experiments according to established protocols, apply fundamental biochemical laboratory techniques, adhere to laboratory safety regulations, analyse and interpret experimental data, and document their findings in laboratory reports. Last update: Křížová Ivana (21.07.2026)
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Bioengineering, Microbiology Last update: Branská Barbora (25.04.2025)
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Basic knowledge of bioengineering and microbiology is assumed. Last update: Branská Barbora (14.05.2025)
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| Teaching methods | ||||
| Activity | Credits | Hours | ||
| Konzultace s vyučujícími | 0.7 | 20 | ||
| Účast v laboratořích (na exkurzi nebo praxi) | 2 | 56 | ||
| Obhajoba individuálního projektu | 0.2 | 6 | ||
| Účast na přednáškách | 0.5 | 14 | ||
| Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 1.3 | 36 | ||
| Práce na individuálním projektu | 1.3 | 36 | ||
| Účast na seminářích | 1 | 28 | ||
| 7 / 7 | 196 / 196 | |||
| Coursework assessment | |
| Form | Significance |
| Defense of an individual project | 20 |
| Report from individual projects | 80 |
