SubjectsSubjects(version: 949)
Course, academic year 2023/2024
Molecular genetics and DNA analysis - AB320015
Title: Molecular genetics and DNA analysis
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2023
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
Teaching methods: full-time
For type: Bachelor's
Guarantor: Ruml Tomáš prof. Ing. CSc.
Interchangeability : B320015, N320081, S320081
Is interchangeable with: B320015
This subject contains the following additional online materials
Annotation -
Last update: Kubová Petra Ing. (06.05.2019)
The subject is focused on understanding heredity principles especially mechanisms and regulation of transfer of genetic information and gene expression in prokaryotic and eukaryotic organisms. Special emphasis will be put on the main regulatory processes mediated by proteins and RNA regulatory molecules. Other focus is on understanding the variability of genetic information due to mutations and recombination. The subject offers information on the most important methods of DNA analysis as DNA identification and sequencing. The students will learn basic basic principles of pharmacogenomics and oncogenesis.
Aim of the course -
Last update: Kubová Petra Ing. (06.05.2019)

Students will know:

Basic principles of molecular genetics and the relationship of their structure and function.

Principles of regulation of transfer of genetic information and its variability due to mutations and recombination

Mechanisms involved in mutation repair.

Basic processes of gene expression as regulation of transcription, in prokaryotes and eukaryotes and posttranscriptional modifications of eukaryotic mRNA, regulation of stability of mRNA and translation, role of small RNAs.

Basic principles of DNA analysis and basic applications of polymerase chain reaction

Basic principles of pharmacogenomics and oncogenesis

Literature -
Last update: Ruml Tomáš prof. Ing. CSc. (15.09.2023)

Michael J Simmons, Peter D. Snustad: Principles of Genetics, 7th edition, Wiley 2016

Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon and Kelsey C. Martin: Molecular Cell Biology 9th Edition, W. H. Freeman, 2021

Learning resources -
Last update: Kubová Petra Ing. (06.05.2019)

Berk A., Lodish H., Kaiser Ch. A.: (2016) Molecular Cell Biology, 8th Edition, Palgrave Macmillan

Syllabus -
Last update: Kubová Petra Ing. (06.05.2019)

1. Historic overview and basic genetic laws and terms.

2. Structure and function of nucleic acids, genome organization

3. Replication, DNA polymerases, replisome complex.

4. Mutations and repair mechanisms.

5. Recombination, gene segregation.

6. Transposition, transduction, regulation of yeast mating type.

7. Regulation of prokaryotic transcription.

8. Eukaryotic transcription, posttranscriptional modifications of RNA.

9. Regulatory roles of RNA.

10. Translation.

11. Comparison of prokaryotic and eukaryotic translation, post-translational modification.

12. DNA analysis for identification of persons.

13. Fundamentals of pharmacogenomics.

14. Genetic principles of cancer.

Entry requirements -
Last update: Kubová Petra Ing. (06.05.2019)

To enroll in the course it is required enrollment in courses N110004 A N320004

Registration requirements -
Last update: Kubová Petra Ing. (06.05.2019)

Biochemistry I

Course completion requirements -
Last update: Kubová Petra Ing. (06.05.2019)

completed written and oral exams