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The course is focused on the principles and advanced applications of modern isolation and separation techniques including representative detection methods. Methods are discussed with regard to practical aspects and problems that are normally encountered. Framework of studied methods: isolation methods in gaseous, liquid, supercritical and solid phase; gas and liquid chromatography, including conventional detectors; mass spectrometry.
Last update: Kubová Petra (30.01.2018)
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Successful completion of a written examination. Last update: Kubová Petra (30.01.2018)
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Pawliszyn J.: Solid phase microextraction: Theory and Practice. Wiley-VCH, Inc. NY, USA, ISBN 0-471-19034-9, 1997. Dean, J.R.: Extraction Methods for Environmental Analysis, J.Wiley&Sons, Ltd., Chichester, Anglie, ISBN 0-471-98287-3, 1998. Grob, R.L. (Editor): Modern Practice of Gas Chromatography, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-59700-7, 1995. Willoughby, R., Sheehan, E., Mitrovich, S.: A Global View of LC/MS: How to Solve Your Most Challenging Analytical Problems, Global View Publishing, Pittsburg, Pennsylvania, USA, ISBN 0-9660813-0-7, 1998. Snyder,L.R., Kirkland, J.J., Glajch, J.L.: Practical HPLC Method Development, J.Wiley&Sons, Inc., NY, USA, ISBN 0-471-00703-X, 1997. Scientific papers. Last update: Poustka Jan (26.06.2024)
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1. General characteristics of analytical procedure and classification of techniques 2. Purge & trap, head space, supercritical fluid extraction 3. Liquid-liquid extraction, liquid micro-extraction 4. Solid-liquid extraction, various means of implementation (ASE-PLE, MASE) 5. Solid phase extraction 6. Solid phase micro-extraction 7. Adsorption chromatography, dispersive solid phase extraction (MSPD, QuEChERS) 8. Gel permeation chromatography 9. Gas chromatography - factors influencing separation, injection techniques 10. Gas chromatography - columns, detectors 11. Gas chromatography - large volume injection; GCxGC 12. Liquid chromatography - factors influencing separation 13. Liquid chromatography - columns, detectors 14. Mass spectrometry Last update: Kubová Petra (30.01.2018)
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https://e-learning.vscht.cz/ Last update: Poustka Jan (13.09.2023)
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Students will be able to understand: 1. Principles, technical configurations and application potential of sample preparation methods 2. Principles, technical configurations and application potential of gas and liquid chromatography including the application of conventional detectors 3. Principles, technical configurations and application potential of mass spectrometric methods based on various principles of ion separation Last update: Kubová Petra (30.01.2018)
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No requirements Last update: Kubová Petra (30.01.2018)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Konzultace s vyučujícími | 0.5 | 14 | ||
Účast na přednáškách | 1.5 | 42 | ||
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 0.5 | 14 | ||
Příprava na zkoušku a její absolvování | 1.5 | 42 | ||
4 / 4 | 112 / 112 |