Isolation and Separation Methods - AM323010
Title: Isolation and Separation Methods
Guaranteed by: Department of Food Analysis and Nutrition (323)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2023
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:3/0, Ex [HT]
Capacity: unlimited / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: English
Teaching methods: full-time
Teaching methods: full-time
Level:  
Additional information: https://e-learning.vscht.cz/
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Poustka Jan prof. Dr. Ing.
Pulkrabová Jana prof. Ing. Ph.D.
Interchangeability : M323010, N323023, S323023
Is interchangeable with: M323010
Examination dates   
This subject contains the following additional online materials
Annotation -
The course is focused on the principles and advanced applications of modern isolation and separation techniques including representative detection methods. Methods are discussed with regard to practical aspects and problems that are normally encountered. Framework of studied methods: isolation methods in gaseous, liquid, supercritical and solid phase; gas and liquid chromatography, including conventional detectors; mass spectrometry.
Last update: Kubová Petra (30.01.2018)
Aim of the course -

Students will be able to understand:

1. Principles, technical configurations and application potential of sample preparation methods

2. Principles, technical configurations and application potential of gas and liquid chromatography including the application of conventional detectors

3. Principles, technical configurations and application potential of mass spectrometric methods based on various principles of ion separation

Last update: Kubová Petra (30.01.2018)
Course completion requirements -

Successful completion of a written examination.

Last update: Kubová Petra (30.01.2018)
Literature -

Recommended:

  • Solid Phase Microextraction, Theory and Practice - SPME, Pawliszyn, Janusz, 1997

  • Extraction Methods for Environmental Analysis, Dean, J.R., 1998

  • Modern practice of gas chromatography, Grob, Robert Lee., 1995

  • A Global View of LC/MS: How to Solve Your Most Challenging Analytical Problems, Willoughby, R., Sheehan, E., Mitrovich, S., 1998

  • Practical HPLC method development, Snyder, Lloyd R., Kirkland, Joseph J., Glajch, Joseph L., 1997

Last update: Poustka Jan (26.06.2024)
Requirements to the exam -

Within the extent of the syllabus.

Last update: Vlčková Martina (02.02.2018)
Syllabus -

1. General characteristics of analytical procedure and classification of techniques

2. Purge & trap, head space, supercritical fluid extraction

3. Liquid-liquid extraction, liquid micro-extraction

4. Solid-liquid extraction, various means of implementation (ASE-PLE, MASE)

5. Solid phase extraction

6. Solid phase micro-extraction

7. Adsorption chromatography, dispersive solid phase extraction (MSPD, QuEChERS)

8. Gel permeation chromatography

9. Gas chromatography - factors influencing separation, injection techniques

10. Gas chromatography - columns, detectors

11. Gas chromatography - large volume injection; GCxGC

12. Liquid chromatography - factors influencing separation

13. Liquid chromatography - columns, detectors

14. Mass spectrometry

Last update: Kubová Petra (30.01.2018)
Learning resources -

https://e-learning.vscht.cz/

Last update: Poustka Jan (13.09.2023)
Registration requirements -

No requirements

Last update: Kubová Petra (30.01.2018)
Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0.5 14
Účast na přednáškách 1.5 42
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0.5 14
Příprava na zkoušku a její absolvování 1.5 42
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