SubjectsSubjects(version: 949)
Course, academic year 2019/2020
Down-stream Processing in Biotechnology - AP319004
Title: Down-stream Processing in Biotechnology
Guaranteed by: Department of Biotechnology (319)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019
Semester: both
Points: 0
E-Credits: 0
Examination process:
Hours per week, examination: 3/0, other [HT]
Capacity: winter:unknown / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
Teaching methods: full-time
For type: doctoral
Note: course is intended for doctoral students only
can be fulfilled in the future
you can enroll for the course in winter and in summer semester
Guarantor: Melzoch Karel prof. Ing. CSc.
Interchangeability : P319004
Examination dates   Schedule   
Annotation -
Last update: Pátková Vlasta (15.11.2018)
Separation processes are an integral part of any industrial biotechnology. Each biotechnological process requires operations that involve separation, concentration, or purification of substances, both raw materials (upstream processes) and products (downstream operations). Students will get acquainted with principles of selected separation processes in biotechnology, their mass and enthalpy balance, process kinetics, instrumentation and equipment used in laboratory scale and industrial practice, including the possibilities of control and modeling of unit operations. It is shown what role strategies and economics play in choosing separation processes and overall purification sequence. Examples of biotechnology from practice are used for demonstration of the realization of separation processes, including the use of integrated processes - the connection of bioprocess with "downstream" techniques.
Aim of the course -
Last update: Pátková Vlasta (15.11.2018)

Students will expand thier knowledge of industrial biotechnology and bioengineering regarding separation processes. In particular, they will acquire skills in the field of upstream and downstream processing of biotechnological production, development, design and optimization of biotechnological processes, including the design of an efficient sequence of separation processes from the point of view of economic indicators, functionality and safety with respect to good manufacturing practice.

Literature -
Last update: Pátková Vlasta (15.11.2018)

Harrison R. G., Todd P., Rudge S. R., Petrides D. P. (2003): Bioseparations Science and Engineering. Oxford University Press, New York,Oxford. ISBN: 0-19-512340-9

Wang W. K. (2001): Membrane Separations in Biotechnology. 2. vyd., Marcel Dekker, Inc., New York, Basel. ISBN: 0-8247-0248-4

Fellows P. J. (2009): Food Processing Technology, Principles and Practice. 3. vyd., Woodhead Publishing Ltd, Cambridge a CRC Press, Boca Raton. ISBN: 978-1-84569-216-2

And series of electronical publications which are available at the web page:

Teaching methods -
Last update: Pátková Vlasta (15.11.2018)

Introductory lectures and subsequent seminars, individual processing of selected topics and their presentation.

Requirements to the exam -
Last update: Pátková Vlasta (15.11.2018)


Syllabus -
Last update: Pátková Vlasta (15.11.2018)
  • Biotechnological production as a sequence of operations - from substrate to product. Selection of the separation process.
  • Membrane processes - microfiltration, ultrafiltration, nanofiltration, reverse osmosis
  • Membrane distillation, pervaporation, gas and vapor permeation, pertraction
  • Electromigration processes, electrophoretic methods, electrodialysis, membrane electrolysis
  • Supercritical fluid extraction, aqueous two-phase systems, biopolymer extraction,
  • Preparative chromatography - adsorption chromatography, molecular sieve chromatography, ion exchange chromatography, hydrophobic interaction chromatography, reverse phase chromatography, affinity chromatography - biospecific interactions
  • Bioprocess integration with up- and down-stream techniques. Membrane bioreactors - Industrial applications.
  • Strategy and economics in choosing the separation technique and the purification sequence
  • Fundamentals of modeling of separation processes

Entry requirements -
Last update: Pátková Vlasta (15.11.2018)

Life science or technical education

Registration requirements -
Last update: Pátková Vlasta (15.11.2018)


Course completion requirements -
Last update: Pátková Vlasta (15.11.2018)

Defense of an individual project on the selected topic. Oral exam