SubjectsSubjects(version: 855)
Course, academic year 2019/2020
  
Special Enzymology - AP320004
Title: Special Enzymology
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2019
Semester: both
Points: 0
E-Credits: 0
Examination process:
Hours per week, examination: 3/0 other [hours/week]
Capacity: winter:unknown / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
Level:  
For type: doctoral
Additional information: http://web.vscht.cz/~spiwokv/enzymologie/
Note: course is intended for doctoral students only
can be fulfilled in the future
you can enroll for the course in winter and in summer semester
Guarantor: Valentová Olga prof. RNDr. CSc.
Spiwok Vojtěch doc. Ing. Ph.D.
Janda Martin Ing. Ph.D.
Interchangeability : D320003, P320004
Annotation -
Last update: Valentová Olga prof. RNDr. CSc. (01.08.2018)
This course focuses on kinetics and mechanisms of enzymatic reactions, thermodynamics of enzyme-inhibitor (or enzyme-cofactor, enzyme-activator etc) interactions, analytical applications and biophysical methods for enzyme assays, 3D structure and mechanisms of their action. The course also focuses on enzyme engineering approaches and enzyme prospection by gene technologies. Properties of selected industrial enzymes will be presented. Enzymes used in molecular biology will be also discussed. In individual projects the students will study a selected type of enzymes, their structure, mechanism, function and application. The choice will follow the topic of the doctoral project or the current state of research in enzymology. The results will be presented in the form of short presentation.
Aim of the course -
Last update: Valentová Olga prof. RNDr. CSc. (31.07.2018)

Students will have understanding of enzyme mechanism, enzyme kinetics and enzyme activity regulation. They will gain knowledge of the technologically important enzymes, their properties and targeted possibilities to discover enzymes with improved properties. Students will be able to design and apply advanced methods for characterization and application of enzymes in biochemical research, drug development, food and noon-food technologies and genetic engineering.

Literature -
Last update: Valentová Olga prof. RNDr. CSc. (01.08.2018)

A:A.G. Marangoni (Ed.): Enzyme Kinetics. John Wiley & Sons, Inc. 2003, ISBN: 9780471159858 (printed), 9780471267294 (online)

A:H. Bisswanger: Enzyme Kinetics. Wiley-VCH Verlag GmbH, 2002, ISBN: 9783527303434 (printed), 9783527601752 (online)

A:R.J. Whitehurst, M. van Oort: Enzymes in Food Technology, Blackwell Publishing Ltd, 2010, ISBN: 9781405183666 (printed), 9781444309935 (online)

A:J. Polaina, A.P. MacCabe: Industrial Enzymes : Structure, Function and Applications, Springer Dordrecht NLD, 2007, ISBN: 9789048173549 (printed), 9781402053771 (online)

A:J.R. Whitaker, A.G.J. Voragen, D.W.S. Wong, Handbook of Food Enzymology, CRC Press 2002 ISBN: 9780824706869 (printed), 9780203910450, (online)

A:W.Aehle: Enzymes in Industry, Wiley-VCH 2004 ISBN 9783527295920 (printed) 9783527602131 (online)

Learning resources -
Last update: Pátková Vlasta (08.06.2018)

http://web.vscht.cz/spiwokv/enzymologie/

http://biomikro.vscht.cz/vyuka/?Predmet=ae

Syllabus -
Last update: Valentová Olga prof. RNDr. CSc. (31.07.2018)

Content of this subject is divided into three blocks the first of which will be focused on the basic characteristics of enzymes, i.e. a) detailed description of enzyme kinetics of single- or multiple-substrate reactions b) enzyme inhibition and c) determination of enzyme activity by the application of spectrophotometric, separatory, electrochemical and other techniques. In the second block students will be acquainted with a) mechanisms of enzyme reactions and approaches to their research and b) modes of regulation of enzyme activity on the molecular level, i.e. by non-covalent interactions and covalent modifications. Third block will be dedicated to the possibilities of enzyme application with respect to a) development of new enzymes by the methods of enzyme engineering, directed evolution, rational design, metagenomic approach etc. b) sources and production of technologically important enzymes and enzymes used in medicine c) characterization of selected enzymes and d) characterization of enzymes applied genetic engineering and molecular biology.

Registration requirements -
Last update: Pátková Vlasta (08.06.2018)

None.

Course completion requirements -
Last update: Pátková Vlasta (08.06.2018)

Written and oral exam, presentation.

Coursework assessment
Form Significance
Report from individual projects 40
Examination test 30
Oral examination 30

 
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