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Last update: Fukal Ladislav prof. Ing. CSc. (05.11.2018)
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Last update: Kubová Petra Ing. (26.06.2018)
Students will be able to:
Understand the function of the immune system, especially with regard to the possibility of influencing the production of antibodies. Parameters of antigen-antibody interaction, the details of the various immunoassay principles. |
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Last update: Kubová Petra Ing. (26.06.2018)
Daussant J., Desvaux F.X., Introduction to Immunochemical Techniques for Medical Diagnosis, Food Quality Control and Environmental Testing, Praha, 2007, ISBN 978-80-7080-641-8 Wild D.: The Immunoassay Handbook – Theory and applications of ligand binding, ELISA and related techniques. Elsevier Ltd. 2013, Oxford, UK. ISBN: 978-0-08-097037-0 Murphy K.: Janewayś ImmunoBiology. GarlandScience New York 2011, ISBN 978-0-8153-4243-4 |
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Last update: Kubová Petra Ing. (26.06.2018)
Daussant J., Desvaux F.X., Introduction to Immunochemical Techniques for Medical Diagnosis, Food Quality Control and Environmental Testing, Praha, 2007, ISBN 978-80-7080-641-8 Wild D.: The Immunoassay Handbook – Theory and applications of ligand binding, ELISA and related techniques. Elsevier Ltd. 2013, Oxford, UK. ISBN: 978-0-08-097037-0 Murphy K.: Janewayś ImmunoBiology. GarlandScience New York 2011, ISBN 978-0-8153-4243-4 |
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Last update: Fukal Ladislav prof. Ing. CSc. (05.11.2018)
Lectures will be focused on the basic functions of individual components of immune system; adaptive part of the immune system (interaction of cell components leading to the final production of specific antibodies); characteristics related to the structure and properties of antigens and antibodies; methods of antibody preparation; parameters antigen-antibody interaction in-vitro; haptens and immunoassay; chemical modification of molecules of antigens and antibodies for immunoassays needs; details about principles and procedures of basic immunochemical methods (precipitation vs. non-precipitation methods, methods with a mark vs. without a mark); Enzyme Immunoassay and differences in variations of its arrangement; non-precipitation immunoassay with other marker types (than enzyme); amplification of the label signal per unit of antibody-antigen interaction; principles of multidetection antibody chips, immunosensors including microfluidic arrangements and surface plasma resonance; immunoblotting, immunohistochemical applications (including fluorescence multiplex), fluorescence flow cytometry. Immuno-affinity chromatography, user-friendly immunochemical assay formats.
Part of the course will be devoted to elaboration and presentation of individual professional projects of students. |
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Last update: Kubová Petra Ing. (26.06.2018)
Passing the course "Laboratory of immunochemical methods". |
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Last update: Kubová Petra Ing. (26.06.2018)
Developing and defending a project. To pass an exam. |