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Last update: Lipov Jan doc. Ing. Ph.D. (02.11.2018)
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Last update: Pátková Vlasta (08.06.2018)
Student will be introduced to modern facts and methodology in advanced microscopy of biological objects, she/he will obtain an overview of possible techniques, which allows her/him more qualified decisions in scientific research activities. |
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Last update: Pátková Vlasta (08.06.2018)
Scientific papers and online books from the portfolio of Nature Research, AAAS and others. |
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Last update: Pátková Vlasta (08.06.2018)
Lectures, professional debate, selflearning, excursion. |
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Last update: Lipov Jan doc. Ing. Ph.D. (02.11.2018)
Fluorescence microscopy as a specific part of optical microscopy, quantification of fluorescence, fluorescent tags. Confocal, multiphoton and correlation microscopy. The application of fluorescence microscopy - FRAP (Fluorescence Recovery After Photobleaching), FRET (Förster Resonance Energy Transfer) The application of fluorescence microscopy - TIRF (Total Internal Reflection Fluorescence), SIM (Structured Illumination Microscopy) The application of fluorescence microscopy - PALM (PhotoActivated Localization Microscopy), STORM (Stochastic Optical Reconstruction Microscopy) The application of fluorescence microscopy - STED (Stimulated Emission Depletion Microscopy), FLIM (Fluorescence-Lifetime Imaging Microscopy). Image analysis – parameter ajdustment before acquisition, software for image processing and analysis – deconvolution, 3D visualization, colocalization, tracking, correction of chromatic abberations. |
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Last update: Pátková Vlasta (08.06.2018)
Basic knowledge in the field of optics and microscopic techniques. |
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Last update: Pátková Vlasta (08.06.2018)
Active presence of the student on the lectures. More then 50% from the final test. |