SubjectsSubjects(version: 941)
Course, academic year 2019/2020
Biochemical Methods - AP320009
Title: Biochemical Methods
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019 to 2021
Semester: both
Points: 0
E-Credits: 0
Examination process:
Hours per week, examination: 3/0, other [HT]
Capacity: winter:unknown / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
Language: English
Teaching methods: full-time
Teaching methods: full-time
For type: doctoral
Note: course is intended for doctoral students only
can be fulfilled in the future
you can enroll for the course in winter and in summer semester
Guarantor: Lipovová Petra doc. Ing. Ph.D.
Káš Jan prof. Ing. DrSc.
Novotná Zuzana doc. Dr. Ing.
Interchangeability : D320002, P320009
Examination dates   Schedule   
Annotation -
Last update: Pátková Vlasta (15.11.2018)
The aim of this course is to deepen the knowledge of the principles of particular biochemical techniques, whether classical or new. Part of this course will be devoted to the extension of knowledge gained in previous studies. In the second part of the course the students will present the latest techniques published in a renowned magazines. In general, the course is designed so that the graduates acquire both the key theoretical knowledge in the field and an overview of the current methodologies used in the biochemical and molecular-biological research.
Aim of the course -
Last update: Pátková Vlasta (15.11.2018)

During the previous master's study students met with theoretical foundations of biochemical methods. Within this subject, their knowledge about the latest approaches and modern methodologies used in laboratory practice will be extended. Students will acquire theoretical and directly practical knowledge and skills in modern biochemical methods. It will also benefit from the enhancement of presentation skills, in which students will prepare a brief briefing on the new techniques they use in the laboratories as part of their dissertation work.

Literature -
Last update: Pátková Vlasta (15.11.2018)

Boyer, Rodney F: Biochemistry laboratory: modern theory and techniques, Pearson, New Jersey, 2012 ISBN-13:978-0-13-604302-7

Janson JC: Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition, John Wiley & Sons, Inc., New Jersey 2011 ISBN:9780471746614

Mikkelsen S.R.: Bioanalytical Chemistry, Wiley, New Jersey, 2016, ISBN 978-1-118-30254-5

Syllabus -
Last update: Pátková Vlasta (15.11.2018)

The first block of lectures will focus on classical methods - work with biological material (storage, homogenization, extraction, sample preparation for mass spectrometry), electrophoretic methods, chromatographic methods, membrane processes, centrifugation, PCR methods, methods of protein interaction analysis - depending on the students' knowledge (lectures and discussions).

The second block will be devoted to the recent techniques, students will present a short presentation of a selected method published in a renowned magazines to other participants of the course.

Entry requirements -
Last update: Pátková Vlasta (15.11.2018)


Registration requirements -
Last update: Pátková Vlasta (15.11.2018)


Course completion requirements -
Last update: Pátková Vlasta (15.11.2018)

Presentation of a review paper form the field of biochemical methods.

Oral examThe subject is finished by an oral exam.