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The course is focused on the principles and advanced applications of modern isolation and separation techniques including representative detection methods. Methods are discussed with regard to practical aspects and problems that are normally encountered. Framework of studied methods: isolation in gaseous, liquid, supercritical and solid phase; gas and liquid chromatography, including conventional detectors, mass spectrometry.
Last update: Pátková Vlasta (15.11.2018)
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Students will be able to understand:
1. Principles, technical configurations and application potential of sample preparation methods
2. Principles, technical configurations and application potential of gas and liquid chromatography including the application of conventional detectors
3. Principles, technical configurations and application potential of mass spectrometric methods based on various principles of ion separation Last update: Pátková Vlasta (15.11.2018)
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Exam - written test. Last update: Pátková Vlasta (15.11.2018)
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Lectures, consultations, self-study. Last update: Pátková Vlasta (15.11.2018)
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Within the extent of the syllabus. Last update: Poustka Jan (13.09.2023)
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1. Overview of analytical methods and techniques in analytical procedures 2. Isolation methods in gas phase - purge & trap (PT), head space (HS) 3. Supercritical fluid extraction (SFE) 4. Liquid extraction – techniques: liquid-liquid extraction (LLE), liquid-solid extraction (LSE) 5. Liquid extraction at elevated temperature and pressure (ASE-PLE, MASE) 6. Solid phase extraction (SPE); solid phase micro-extraction (SPME) 7. Adsorption chromatography (LSC); gel permeation chromatography (GPC) 8. Dispersive solid phase extraction (MSPD, QuEChERS) 9. Gas chromatography – parameters; separation process, injection techniques; columns, detectors; GCxGC 10. Liquid chromatography – parameters; separation process, columns, detectors 11. Mass spectrometry – ionisation; mass analyzers, spectra interpretation Last update: Pátková Vlasta (15.11.2018)
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https://e-learning.vscht.cz/ Last update: Poustka Jan (13.09.2023)
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Basic knowledge of the fields: general chemistry, inorganic chemistry, organic chemistry, analytical chemistry. Last update: Pátková Vlasta (15.11.2018)
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No requirements. Last update: Pátková Vlasta (15.11.2018)
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