SubjectsSubjects(version: 963)
Course, academic year 2024/2025
  
Microscopy of Materials - B107016
Title: Mikroskopie materiálů
Guaranteed by: Department of Glass and Ceramics (107)
Faculty: Faculty of Chemical Technology
Actual: from 2024
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:2/1, C+Ex [HT]
Capacity: unlimited / unlimited (unknown)
Min. number of students: unlimited
State of the course:  
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Level:  
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Macháček Jan Ing. Ph.D.
Kolářová Mária Ing. Ph.D.
Polonská Adéla Ing. Bc. Ph.D.
Examination dates   Schedule   
Annotation -
The aim of the course is to teach the student to master microscopic techniques at an advanced level and to understand their principles. The subject is suitable for preparing the student for the part of his bachelor's thesis that concerns the characterization of samples. In addition, the knowledge gained in the subject is also suitable for solving tasks from production practice that require the use of microscopy. First of all, it concerns microscopic analyzes of manufacturing defects.
Last update: Macháček Jan (19.02.2024)
Course completion requirements -

The credit is awarded for attendance.

The exam consists of a written and oral part.

Last update: Macháček Jan (23.02.2024)
Literature -

R: Bartuška M: Technická mineralogie, skripta, VŠCHT Praha, 1987.

R: Gregerová M, Fojt B, Vávra B: Mikroskopie horninotvorných a technických minerálů, Morav. zem. muz., PřF MU Brno, 2002.ISBN:80-7028-195-2

A: Mackenzie WS, Guilford C: Atlas of rock-forming minerals in thin section, Longman, 1980.ISBN-10:0470269219,ISBN-13:978-0470269213

A: Raith MM, Raase P, Reinhardt J: Guide to thin section microscopy, 2011.ISBN:978-3-00-033606-5

R: Russ JC: The image processing handbook, CRC Press, 2007. ISBN-10:1439840458,ISBN-13:978-1439840450

A: Wu Q, Merchant F, Castleman KR: Microscope Image Processing, Elsevier, 2008.ISBN-10:012372578X,ISBN-13:978-0123725783

Last update: Macháček Jan (23.02.2024)
Requirements to the exam - Czech

písemná i ústní zkouška

Last update: Macháček Jan (19.02.2024)
Syllabus -

1. Basic concepts of optics: light as an electromagnetic wave – index of refraction – reflection and refraction of light – polarization of light – interference of light – diffraction of light – geometrical optics – optical defects.

2. Advanced optics: propagation of light in anisotropic substances - use of birefringent substances - photometry - colorimetry.

3. Classical (light) microscopy – imaging principles – lateral resolution – depth of field – microscope components – microscopy technique – reflection microscopy – specimen preparation – microscope maintenance.

4. Polarization microscopy: imaging principles, parallel polarizers (PPL) – crossed polarizers (XPL) – compensators – conoscopic imaging – reflection microscopy in XPL.

5. Evaluation of optical properties of crystals in polarized light: microstructure and texture - relief - measurement of refractive index - pleochroism.

6. Evaluation of the optical properties of crystals in crossed polarizers: extinction – interference colors and specific birefringence – character of the zone and optical character of the mineral – measurement of stress in the material.

7. Digital microscopy and contrast enhancement: digital microscopy - dynamic range of imaging (HDR) - extended depth of field (EDF) - dark field - phase contrast - differential interference contrast (DIC) - interference microscopy.

8. Confocal microscopy: imaging principles – lateral and axial resolution – microscope components – microscopy technique – height map processing – roughness and waviness parameters – layer thickness measurement – particle size measurement – volume measurements.

9. Special microscopy: fluorescence microscopy – Raman and IR microscopy – microhardness measurement – high temperature microscopy – tomography and 3D scanning.

10. Microscopic image analysis: digital imaging and image processing – image segmentation – methods of mathematical morphology – determination of particle size distribution – stereology – image segmentation using artificial intelligence.

11. Specifics of microscopy of materials: microscopy of raw materials and rocks – ceramic materials – metal materials and biomaterials – polymer materials and fibers – defects in glass and stresses in the material.

12. Scanning electron microscopy in the context of material defect analysis.

13. Elemental analysis in electron microscopy (EDX) and light microscopy (LIBS).

14. Summary of the strategy of microscopic analysis of materials.

Last update: Macháček Jan (23.02.2024)
Learning resources -

micro.magnet.fsu.edu/primer

www.olympusmicro.com

petrol.sci.muni.cz/mikroskopie/uvod.htm

www.earth.ox.ac.uk/~oesis/micro

webmineral.com

Last update: Macháček Jan (23.02.2024)
Registration requirements -

prerequisites - none

co-requisites - none

equivalence - none

Last update: Macháček Jan (23.02.2024)
 
VŠCHT Praha