SubjectsSubjects(version: 955)
Course, academic year 2023/2024
Ecotoxicology Laboratory - B240009
Title: Laboratoř ekotoxikologie
Guaranteed by: Department of Environmental Chemistry (240)
Faculty: Faculty of Environmental Technology
Actual: from 2021
Semester: summer
Points: summer s.:6
E-Credits: summer s.:6
Examination process: summer s.:
Hours per week, examination: summer s.:0/8, MC [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Guarantor: Vurm Radek Ing. Ph.D.
Class: Základní laboratoře
Interchangeability : N240017
Is interchangeable with: AB240009
Annotation -
The transfer of theoretical knowledge of the field of ecotoxicology into practice. Students will learn the foundation of selected ecotoxicological tests on organisms of different trophic levels (decomposers, producers, consumers) terrestrial and aquatic environments, methods of evaluation and statistical analysis of the results and their subsequent interpretation.
Last update: Vurm Radek (19.01.2018)
Aim of the course -

Students will be able to:

  • do basic techniques of preparation organisms and obtain basic skill of microscopy
  • perform basic ecotoxicological tests with aquatic and terrestrial organisms (microorganisms, aquatic crustaceans, aquatic macrophytes, algae, soil invertebrates, terrestrial plants)
  • perform statistical evaluation and interpretation of ecotoxicological data
  • evaluate protocols on ecotoxicological issues.

Last update: Vurm Radek (19.01.2018)
Literature -

R: Instructions for the preparation labs available on the website of the Working Group.

Last update: Vurm Radek (26.01.2018)
Learning resources -

Last update: Vurm Radek (26.01.2018)
Requirements to the exam -

The labs are designed as a 14-day batch. The exact date will be announced before the beginning of the semester.

Attendance is mandatory. After exercise students will work out protocols of individual laboratory works within the specified time.

Last update: Vurm Radek (19.01.2018)
Syllabus -

1. Preparation and pre-treatment of samples for toxicity tests.

2. Working with a microscope

3. Working in a sterile box.

4. Breeding and cultivation conditions of the test organisms.

5. Planning of experiments, the choice of the concentration series, statistical evaluation of data.

6. Test of acute toxicity to aquatic crustaceans (Daphnia magna).

7. Test on brine shrimp (Artemia salina).

8. Growth inhibition test on chlorococcal algae (Desmodesmus subspicatus).

9. Growth inhibition test of aquatic macrophytes - duckweed (Lemna minor).

10.Terrestrial test - inhibition of root growth of lettuce (Lactuca sativa).

11.Test of inhibition of dehydrogenase activity of soils.

12.Behavioral test with soil invertebrates.

13.Bioluminiscence inhibition test on Vibrio fischeri.

14.Protocols processing and comparison of results

Last update: Vurm Radek (26.01.2018)
Registration requirements -


Last update: Vurm Radek (26.01.2018)
Course completion requirements -

Students have to graduate all laboratory works and consign protocols from these works.

Last update: Vurm Radek (26.01.2018)
Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0.4 10
Účast v laboratořích (na exkurzi nebo praxi) 4.8 135
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0.8 23
6 / 6 168 / 168