SubjectsSubjects(version: 877)
Course, academic year 2020/2021
Fundamentals of Bioengineering - B319002
Title: Základy bioinženýrství
Guaranteed by: Department of Biotechnology (319)
Actual: from 2019
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:2/1 C+Ex [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type: Bachelor's
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Halecký Martin doc. Ing. Ph.D.
Interchangeability : AB319002, N319002
Z//Is interchangeable with: AB319002
Examination dates   Schedule   
This subject contains the following additional online materials
Annotation -
Last update: Kubová Petra Ing. (18.12.2017)
The course focuses on understanding the whole process of industrial bioprocesses e.g. cell cultivation, upstream and downstream processes, process interactions and engineering aspects of the process. The subject is based on expansion, synthesis and application of knowledge gained in basic subjects such as microbiology, biochemistry, physical chemistry and chemical engineering to bioengineering aspects of industrial bioprocesses. The subject brings new or significantly expanding existing knowledge in the field of mass balance bioprocesses, types of bioreactors and methods of management and control of bioprocesses.
Aim of the course -
Last update: Kubová Petra Ing. (18.12.2017)

Students will be able to:

Apply knowledge of basic subjects such as microbiology, biochemistry and chemical engineering to bioengineering area.

Propose a suitable type of cultivation and bioreactor for the desired product.

Design and implement a mass balance and basic process control.

Literature -
Last update: Halecký Martin doc. Ing. Ph.D. (12.01.2018)

R: Najafpour G. D., Biochemical Engineering and Biotechnology, Elsevier B.V., Amsterdam, 2007, ISBN: 978-0-444-52845-2

R: Rehm H.-J., Reed G., Biotechnology, Volume 3 - Bioprocessing WILEY-VCH, 1993, ISBN: 9783527283101

R: Rehm H.-J., Reed G., Biotechnology, Volume 4 - Measuring, Modelling and Control, Second Edition, WILEY-VCH, 1991, ISBN: 9783527283101

Learning resources -
Last update: Halecký Martin doc. Ing. Ph.D. (12.01.2018)

Syllabus -
Last update: Kubová Petra Ing. (18.12.2017)

1. Biotechnology and bioengineering, biotechnological process structure.

2. Technological aspects of use of intact cells as a producers (plant vs. animal vs. microorganisms). Types of products. Application of enzymes.

3. Upstream processes (storage of microorganisms, preparation of inoculum, preparation and sterilization of liquid media, sterilization of reactor, SIP, filtration and sterilization of gases).

4. Parameters for the description of microorganism cultivation. Yield and productivity of process. Overview of cultivation types.

5. Batch cultivation (characteristics, mass balance, use).

6. Fed-batch cultivation (characteristics, types of feeding, mass balance, use).

7. Continuous cultivation (characteristics, process control, mass balance, compared to batch cultivation, use). Choosing the cultivation type on the basis of the desired product.

8. Aeration and oxygen transfer (implementation of aeration, oxygen transfer mechanism, KLa).

9. Mixing (mixing types in biotechnological plant, specifics of mixing in bioreactors).

10. Bioreactors (types, design, use).

11. Measured and controlled variables in cell cultivation. Fundamentals of bioprocess control (controllers, control loop, variables in controlling).

12. Downstream processes (conventional separation technologies, disintegration of cells, membrane separation technologies).

13. Downstream processes (extraction, precipitation, distillation, drying, stripping, CIP). Production line of biotechnological process.

14. Biotechnologies and bioreactors for treatment of wastes from biotechnological processes.

Registration requirements -
Last update: Kubová Petra Ing. (18.12.2017)

Physical chemistry I

Biochemistry I

Course completion requirements -
Last update: Halecký Martin doc. Ing. Ph.D. (31.01.2018)

Credit test; test and oral exam.

Teaching methods
Activity Credits Hours
Účast na přednáškách 1,5 42
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0,9 26
Příprava na zkoušku a její absolvování 1,1 30
Účast na seminářích 0,5 14
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