|
|
|
||
Students will obtain theoretical and practical knowledge of biochemical laboratory techniques, which are commonly used in research work. They will manage basic operations necessary for further routine work in biochemical laboratory. They will try wide spectrum of biochemical techniques, particularly in the area of biomolecules isolation and analysis of biological samples.
Last update: Kubová Petra (04.12.2017)
|
|
||
Students complete all laboratory work, submit all protocols, and successfully pass the final test. Last update: Marková Michaela (25.01.2018)
|
|
||
Návody na úlohy na odkazu: https://e-learning.vscht.cz/course/view.php?id=381 Last update: Podzimek Tomáš (15.09.2023)
|
|
||
1. Introduction 2. Electrophoretic methods (paper and SDS-PAGE electrophoresis) 3. Separation of saccharides and amino acids by thin-layer chromatography 4. Isolation of DNA from pig spleen 5. Spectrophotometric determination of protein and DNA concentrations in a mixed sample 6. Separation of macromolecules by gel filtration chromatography 7. Immunochemical detection of gluten in food samples 8. Separation of lysosyme from egg white (albumen) by ion-exchange chromatography 9. Study of enzyme properties of trypsin using chromogenic substrate 10. Immobilisation of yeasts to alginate gel 11. Titration curves of amino acids 12. Test Last update: Podzimek Tomáš (15.09.2023)
|
|
||
Lab manual and educational videos on link: https://e-learning.vscht.cz/course/view.php?id=1124 Last update: Podzimek Tomáš (15.09.2023)
|
|
||
Students will be able to: use various modern biochemical techniques, which are used in current research. They will learn to use basic biochemical operations such as isolation of selected biomolecules, their quantitation, chromatographical and electrophoretical characterization,kinetic enzyme studies etc. The acquired knowledge will be used by students during their work on bachelor and diploma thesis. Last update: Kubová Petra (04.12.2017)
|
Teaching methods | ||||
Activity | Credits | Hours | ||
Účast v laboratořích (na exkurzi nebo praxi) | 1 | 28 | ||
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 1 | 28 | ||
Příprava na zkoušku a její absolvování | 1 | 28 | ||
3 / 3 | 84 / 84 |