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Last update: Kubová Petra Ing. (04.12.2017)
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Last update: Kubová Petra Ing. (04.12.2017)
Students will be able to: use various modern biochemical techniques, which are used in current research. They will learn to use basic biochemical operations such as isolation of selected biomolecules, their quantitation, chromatographical and electrophoretical characterization,kinetic enzyme studies etc. The acquired knowledge will be used by students during their work on bachelor and diploma thesis. |
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Last update: Podzimek Tomáš Ing. Ph.D. (15.09.2023)
Návody na úlohy na odkazu: https://e-learning.vscht.cz/course/view.php?id=381 |
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Last update: Podzimek Tomáš Ing. Ph.D. (15.09.2023)
Lab manual and educational videos on link: https://e-learning.vscht.cz/course/view.php?id=1124 |
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Last update: Podzimek Tomáš Ing. Ph.D. (15.09.2023)
1. Introduction 2. Electrophoretic methods (paper and SDS-PAGE electrophoresis) 3. Separation of saccharides and amino acids by thin-layer chromatography 4. Isolation of DNA from pig spleen 5. Spectrophotometric determination of protein and DNA concentrations in a mixed sample 6. Separation of macromolecules by gel filtration chromatography 7. Immunochemical detection of gluten in food samples 8. Separation of lysosyme from egg white (albumen) by ion-exchange chromatography 9. Study of enzyme properties of trypsin using chromogenic substrate 10. Immobilisation of yeasts to alginate gel 11. Titration curves of amino acids 12. Test |
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Last update: Marková Michaela Ing. Ph.D. (25.01.2018)
Students complete all laboratory work, submit all protocols, and successfully pass the final test. |
Teaching methods | ||||
Activity | Credits | Hours | ||
Účast v laboratořích (na exkurzi nebo praxi) | 1 | 28 | ||
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 1 | 28 | ||
Příprava na zkoušku a její absolvování | 1 | 28 | ||
3 / 3 | 84 / 84 |