Laboratory of biochemistry - B320005
Title: Laboratoř biochemie
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2020
Semester: both
Points: 3
E-Credits: 3
Examination process:
Hours per week, examination: 0/4 MC [hours/week]
Capacity: winter:unknown / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type: Bachelor's
Note: course can be enrolled in outside the study plan
enabled for web enrollment
you can enroll for the course in winter and in summer semester
Guarantor: Podzimek Tomáš Ing. Ph.D.
Marková Michaela Ing. Ph.D.
Interchangeability : AB320005, N320014
Is interchangeable with: AB320005
Examination dates   
This subject contains the following additional online materials
Annotation -
Last update: Kubová Petra Ing. (04.12.2017)
Students will obtain theoretical and practical knowledge of biochemical laboratory techniques, which are commonly used in research work. They will manage basic operations necessary for further routine work in biochemical laboratory. They will try wide spectrum of biochemical techniques, particularly in the area of biomolecules isolation and analysis of biological samples.
Aim of the course -
Last update: Kubová Petra Ing. (04.12.2017)

Students will be able to:

use various modern biochemical techniques, which are used in current research. They will learn to use basic biochemical operations such as isolation of selected biomolecules, their quantitation, chromatographical and electrophoretical characterization,kinetic enzyme studies etc.

The acquired knowledge will be used by students during their work on bachelor and diploma thesis.

Literature - Czech
Last update: Kubová Petra Ing. (04.12.2017)

Z: Káš J., Kodíček M., Valentová O., Laboratorní techniky biochemie, Praha 2005, ISBN 80-7080-586-2

A: Ruml T., Rumlová M., Pačes V., Genové inženýrství, Praha 2002, ISBN 80-7080-499-8

Learning resources -
Last update: Kubová Petra Ing. (04.12.2017)

Materials from Biochemistry: Seminar I

http://en.wikipedia.org/wiki/Chromatography

http://en.wikipedia.org/wiki/Antibody

http://en.wikipedia.org/wiki/SDS-PAGE

http://en.wikipedia.org/wiki/Dna_extraction

http://en.wikipedia.org/wiki/Spectrophotometry

Syllabus -
Last update: Kubová Petra Ing. (04.12.2017)

1. Introduction

2. Electrophoretic methods (paper and SDS-PAGE electrophoresis)

3. Separation of saccharides and amino acids by thin-layer chromatography

4. Isolation of DNA from pig spleen

5. Spectrophotometric determination of protein and DNA concentrations in a mixed sample

6. Separation of macromolecules by gel filtration chromatography

7. Immunochemical detection of peanut lectin in food samples

8. Separation of lysosyme from egg white (albumen) by ion-exchange chromatography

9. Study of enzyme properties of trypsin using chromogenic substrate

10. Immobilisation of yeasts to alginate gel

11. Titration curves of amino acids

12. Test

Course completion requirements -
Last update: Marková Michaela Ing. Ph.D. (25.01.2018)

Students complete all laboratory work, submit all protocols, and successfully pass the final test.

Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1 28
Příprava na zkoušku a její absolvování 1 28
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