SubjectsSubjects(version: 855)
Course, academic year 2019/2020
  
Bioorganic Chemistry - D110016
Title: Bioorganická chemie
Guaranteed by: Department of Organic Chemistry (110)
Actual: from 2011
Semester: summer
Points: summer s.:0
E-Credits: summer s.:0
Examination process: summer s.:
Hours per week, examination: summer s.:0/0 other [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type:  
Note: course is intended for doctoral students only
can be fulfilled in the future
Guarantor: Hocek Michal prof. Ing. DSc.
Is interchangeable with: AP110009, P110009
Examination dates   Schedule   
Annotation -
Last update: Cibulka Radek prof. Ing. Ph.D. (11.04.2014)
The course of Bioorganic chemistry covers the interdisciplinary area at the interface between organic chemistry and biochemistry or biology. It combines and compares molecular principles of structures of functions of biomolecules, molecular principles of biological and biochemical processes, chemical syntheses and biosyntheses of biomolecules and biomacromolecules and bioconjugations, use of biocatalysis in organic synthesis, and the area of chemical biology (the use of chemical means and approaches for the study of biological processes).
Aim of the course -
Last update: Cibulka Radek prof. Ing. Ph.D. (11.04.2014)

Students will be able to:

understand the structure and function of biomolecules

know molecular principles and reaction mechanisms of biological and biochemical processes

chemical syntřheses of biomolecules and bioconjugates

applications of biocatalysis in chemistry

Literature -
Last update: Cibulka Radek prof. Ing. Ph.D. (04.05.2014)

R: Van Vranken D., Weiss G. A.: Introduction to Bioorganic Chemistry and Chemical Biology, Taylor & Francis Group, 2012. 9780815342144

Learning resources -
Last update: Cibulka Radek prof. Ing. Ph.D. (11.04.2014)

Available from the lecturer (copies of all presentations on CD)

Syllabus -
Last update: Cibulka Radek prof. Ing. Ph.D. (11.04.2014)

1.Introduction: basic principles of molecular interactions in chemistry and biology, short introduction to cellular biology.

2.Structure and functions of nucleic acids: structures of DNA and RNA, genetic information, other functions of RNA, triplexes and quadruplexes, complexes of nucleic acids with proteins, binding of small molecules, DNA damage.

3.Biosynthesis and metabolism of nucleic acids and their components: biosynthesis of DNA (replication) and RNA (transcription) and their further modifications, catabolism of nucleic acids, biosynthesis and catabolism of nucleobases, nucleosides and nucleotides.

4.Chemical and enzymatic syntheses of nucleic acids and their components: chemical synthesis and modifications of nucleobases, nucleosides and nucleotides, chemical synthesis of oligonucleotides on solid-support, biocatalytical synthesis and construction of oligonucleotides and nucleic acids (primer extension, PCR).

5.Structure and functions of proteins: structures of proteins (primary to quaternary), conformations, conjugates (glyco-, phospho-, metalloproteins etc.), isolation and analysis of proteins, structure and function of peptides

6.Biosynthesis and metabolism of proteins: biosyntheses and metabolism of amino acids, biosynthesis of proteins (translation), posttranslation modifications, cleavage and catabolism of proteins, protein engineering, regulation of gene expression

7.Chemical and biotechnological syntheses of peptides and proteins: chemical syntheses of peptides in solution and on solid support, protecting and activating groups, combination of chemical and enzymatic approaches, recombinant method, peptidomimetics, combinatorial approaches

8.Enzymes and mechanisms of enzymatic reactions: enzymes, cofactors, mechanisms and regulation of enzymatic reactions, thermodynamics and kinetics of enzymatic reactions, inhibition

9.Biocatalytical and biotechnological methods in organic synthesis: use of enzymes, antibodies and whole microorganisms and cell cultures in organic synthesis, enzyme engineering and biotechnology

10.Carbohydrates: structure and functions of carbohydrates and oligosaccharides, metabolism and basic principles in chemical syntheses of oligosaccharides and glycosides, saccharide code, synthetic vaccines

11.Membranes and regulation processes: structures and functions of biomembranes, transports of ions and molecules through membranes, regulation processes - hormones, receptors and signal transduction cascades

12.Biosynthesis and metabolism of other classes of natural compounds: metabolism of lipids, terpenoids, alkaloids, polyketides etc., biomimetic total syntheses of natural compounds

13.Chemical biology: use of chemical means in studying and modulation of biological processes, structural analogues of biomolecules (antimetabolites), chemical genetics, construction of artificial bioanalogous systems, principles of in vitro selection. Bioconjugate chemistry.

Course completion requirements -
Last update: Cibulka Radek prof. Ing. Ph.D. (12.05.2014)

It is compulsory to pass written examination test with the result at least 50%.

Coursework assessment
Form Significance
Examination test 100

 
VŠCHT Praha