SubjectsSubjects(version: 947)
Course, academic year 2021/2022
Biochemical Methods - D320002
Title: Biochemické metody
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2006
Semester: both
Points: 0
E-Credits: 0
Examination process:
Hours per week, examination: 0/0, other [HT]
Capacity: winter:unknown / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
For type:  
Note: course is intended for doctoral students only
can be fulfilled in the future
you can enroll for the course in winter and in summer semester
Guarantor: Káš Jan prof. Ing. DrSc.
Annotation - Czech
Last update: SMIDOVAL (19.08.2011)
Metody separace, izolace a charakterizace biomakrotomolekul
Syllabus -
Last update: TAJ320 (03.02.2016)

1. Centrifugation methods (types of centrifuges and their application)

2. Coagulation methods used for protein isolation

3. Separation methods based on molecular size (dialysis, ultrafiltration, chromatographic and electrophoretic methods

4. Chromatographic techniques (survey of different techniques, separation principles, description of their realization, examples of application)

5. Electro migration techniques (survey of techniques, separation principles, description of their realization and examples of application)

6. Immunochemical techniques (techniques types, description of their realization and examples of application)

7. Detection and assays of proteins, saccharides and nucleic acids

8. Spectrophotometric methods (techniques types, description of their realization and examples of application)

9. Methods for investigation of spatial conformation of biomacromolecules ( roentgen structural analysis, nuclear magnetic resonance, circular dichroisms)

10. Mass spectrometry of macromolecules

11. Fluorometry and luminometry (techniques principles and examples of their application)

12. Methods of polymerase chain reactions (principles and application)

13. Methods of the determination of the catalytic activity of enzymes (general principles and concrete examples)

14. Quality of water in biochemical laboratory and testing of its quality