SubjectsSubjects(version: 855)
Course, academic year 2019/2020
  
Enzymology - D320003
Title: Enzymologie
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2014
Semester: summer
Points: summer s.:0
E-Credits: summer s.:0
Examination process: summer s.:
Hours per week, examination: summer s.:0/0 other [hours/week]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type:  
Additional information: http://web.vscht.cz/~spiwokv/enzymologie/
Note: course is intended for doctoral students only
can be fulfilled in the future
Guarantor: Valentová Olga prof. RNDr. CSc.
Spiwok Vojtěch doc. Ing. Ph.D.
Is interchangeable with: AP320004, P320004
Examination dates   Schedule   
Annotation -
Last update: Spiwok Vojtěch doc. Ing. Ph.D. (05.03.2014)
The general part of the course presents advanced topics of enzyme kinetics, enzyme assays with a focus on "high throughput" approaches, regulation of enzymes in the cell, medicinal enzymology, enzyme engineering and mechanisms of enzymatic reactions. The second part will present applied enzymology, namely industrial production of enzymes and current trends in application of enzymes in biotechnology, analytical chemistry, clinical diagnostics and organic synthesis.
Aim of the course -
Last update: Spiwok Vojtěch doc. Ing. Ph.D. (05.03.2014)

Students will be able to:

Design and apply advanced methods for characterisation and application of enzymes in biochemistry research, drug design, food and non-food technologies and analytical chemistry

Literature -
Last update: Spiwok Vojtěch doc. Ing. Ph.D. (05.03.2014)

A:A.G. Marangoni (Ed.): Enzyme Kinetics. John Wiley & Sons, Inc. 2003, ISBN: 9780471159858 (printed), 9780471267294 (online)

A:H. Bisswanger: Enzyme Kinetics. Wiley-VCH Verlag GmbH, 2002, ISBN: 9783527303434 (printed), 9783527601752 (online)

A:R.J. Whitehurst, M. van Oort: Enzymes in Food Technology, Blackwell Publishing Ltd, 2010, ISBN: 9781405183666 (printed), 9781444309935 (online)

A:J. Polaina, A.P. MacCabe: Industrial Enzymes : Structure, Function and Applications, Springer Dordrecht NLD, 2007, ISBN: 9789048173549 (printed), 9781402053771 (online)

A:J.R. Whitaker, A.G.J. Voragen, D.W.S. Wong, Handbook of Food Enzymology, CRC Press 2002 ISBN: 9780824706869 (printed), 9780203910450, (online)

Learning resources -
Last update: Spiwok Vojtěch doc. Ing. Ph.D. (05.03.2014)

http://web.vscht.cz/spiwokv/enzymologie/

http://biomikro.vscht.cz/vyuka/?Predmet=ae

Syllabus -
Last update: Spiwok Vojtěch doc. Ing. Ph.D. (05.03.2014)

1. Enzyme kinetics, Michaelis-Menten kinetics, multi-substrate reactions

2. Enzyme inhibition

3. Enzyme assays, application of optical, separation, electrochemical and other methods

4. Regulation of enzyme activity by non-covalent interactions and covalent modifications, methods of enzyme research

5. Enzymes as drug targets, drug design

6. Explanation of enzyme catalysis, mechanisms of enzymatic reactions, methods of mechanism studies

7. Enzyme engineering, rational and directed evolution

8. Enzyme sources, searching and production of new enzymes

9. Application of enzymes in food and non-food technologies

11. Enzymes as analytical sensors and diagnostic tools

12. Biotransformations, enzymes in organic synthesis

Registration requirements -
Last update: Spiwok Vojtěch doc. Ing. Ph.D. (05.03.2014)

None.

Course completion requirements -
Last update: Spiwok Vojtěch doc. Ing. Ph.D. (05.03.2014)

Written and oral exam, written essay.

Coursework assessment
Form Significance
Regular attendance 20
Examination test 40
Oral examination 40

 
VŠCHT Praha