SubjectsSubjects(version: 922)
Course, academic year 2022/2023
Liquid chromatography in Analysis of Foods and Natural Products - D323016
Title: Kapalinová chromatografie v analýze potravin a přírodních produktů
Guaranteed by: Department of Food Analysis and Nutrition (323)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019
Semester: summer
Points: summer s.:0
E-Credits: summer s.:0
Examination process: summer s.:
Hours per week, examination: summer s.:0/0, other [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type:  
Note: course is intended for doctoral students only
can be fulfilled in the future
Guarantor: Schulzová Věra doc. Dr. Ing.
Tomaniová Monika Ing. Ph.D.
Is interchangeable with: AP323002, P323002
Annotation -
Last update: Schulzová Věra doc. Dr. Ing. (03.03.2014)
The course focuses on the principles of separation by liquid chromatography, the column selection, mobile phase, and detection. Special focus is given to the selection and optimization of chromatographic conditions. Development and validation of analytical methods is discussed in detail. In specific cases the various problems and their solutions are discussed. Attention focusses on the use of liquid chromatography in analysis of foods and natural products (monitoring of biologically active substances) and residual analysis.
Aim of the course -
Last update: Schulzová Věra doc. Dr. Ing. (03.03.2014)

Students will be able to:

  • Understand the basic principles of liquid chromatography
  • Optimize the separation and detection conditions
  • Applied HPLC technique for detection of natural food components and contaminants

Literature -
Last update: Schulzová Věra doc. Dr. Ing. (28.02.2014)


Food Analysis by HPLC - Nollet (2000)

Practical HPLC - Meyer (1996)

Practical HPLC Method development - L.R.Snyder, J.J. Kirkland, J. L. Glajch (1997)

LC/MS a practical users guide - M. C. McMaster (2005)

Pitfalls and Errors of HPLC in Pictures - V. R. Meyer (2006)

electronic book:

HPLC and CE: Principles and Practice

Andrea Weston and Phyllis R. Brown (1997)


Journal of Chromatography


LC-GC International

Journal of Liquid Chromatography


LC Troubleshooting:

HPLC guide;jsessionid=4EAB9422438DB4C4A206CBBE16FD089A?chId=4

High Performance Liquid Chromatography (HPLC): A Users Guide

Practical HPLC Method Development, 2nd Edition (Hardcover)

Links to Various Resources, topics, courses

HPLC Chromatography Books

Basic Liquid Chromatography

HPLC portal

The HPLC troubleshooter

useful HPLC links

e-learning HPLC

Learning resources -
Last update: Schulzová Věra doc. Dr. Ing. (28.02.2014)

Syllabus -
Last update: Schulzová Věra doc. Dr. Ing. (03.03.2014)

1 Introduction, principles of separation, main principles

2 New trends

3 Mobile phases

4 Methods development - optimization of the chromatographic separation process

5 Factors affecting the HPLC determination

6 Problems in HPLC and their solutions

7 Detection - conventional detectors

8 Detection - mass detectors

9 Derivatization techniques, injection techniques

10 Application of HPLC in food analysis

11 Application of LC / MS analysis of food

12 Case studies and their presentation

13 Case studies and their presentation

14 The final test, examination

Entry requirements -
Last update: Schulzová Věra doc. Dr. Ing. (28.02.2014)

Analytical Chemistry

Food Analysis

Course completion requirements -
Last update: Schulzová Věra doc. Dr. Ing. (03.03.2014)

written examination

project presentation and discussion

Coursework assessment
Form Significance
Regular attendance 10
Defense of an individual project 30
Examination test 60