SubjectsSubjects(version: 954)
Course, academic year 2019/2020
Microbiology in Environmental Protection - M240014
Title: Mikrobiologie pro ochranu životního prostředí
Guaranteed by: Department of Environmental Chemistry (240)
Faculty: Faculty of Environmental Technology
Actual: from 2019 to 2020
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:2/2, C+Ex [HT]
Capacity: 8 / 8 (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Chumchalová Jana Dr. Ing.
Fiala Jaromír Ing. Ph.D.
Class: Základní laboratoře
Interchangeability : N240023
Examination dates   Schedule   
Annotation -
The course is directed to the theoretical and practical knowledge dealing with microorganisms in their environment. Lectures will include information on characterisation of microorganisms, degradation of substrates. Special attention will be paid to the influence of environmental conditions on metabolical and physiological activity, microorganism detection and enumeration. Requirements on microbiological laboratory and general instructions for safe microbiological handling will be mentioned in the last part. Part of the subject will be directed to the laboratory experiments. Students will be introduced into application of basic cultivation techniques and rapid methods for detection and characterization of microorganisms in environmental samples.
Last update: Chumchalová Jana (26.01.2018)
Aim of the course -

Students will be able to practically apply the knowledge from the field of:

  • microorganism taxonomy,
  • metabolism and genetic,
  • both theoretical and practical basic laboratory techniques used for determination and characterization of microorganisms.

Last update: Chumchalová Jana (26.01.2018)
Literature -

Pepper I.L., Gerba G.P., Gentry T.J. Environmental Microbiology. 3. vyd. Elsevier, 2015. ISBN 9780123946263

Last update: Chumchalová Jana (26.01.2018)
Learning resources -

Chumchalová J., Kubal M.: Praktická cvičení z environmentální mikrobiologie, VŠCHT Praha, 2017.

Miniatlas mikroorganismů.

Last update: Chumchalová Jana (07.02.2018)
Requirements to the exam -

Rules to grant credit: student will attend laboratories and deliver report from laboratory praxis in term.

Rules to get exam: student will consist within syllabus of a written part and then listener will be interviewed orally.

Last update: Chumchalová Jana (26.01.2018)
Syllabus -

1. Specification of the subject Microbiology in environment, basic microbiological terms, microbiological taxonomy.

2. Basic structure of microbial cells and their chemical content.

3. Metabolical processes in microorganism.

4. Influence of internal and external factors on growth and characteristics of microorganisms.

5. Nutrition of microorganisms. Growth curve.

6. Principles of both detection and enumeration of microorganisms by cultivation techniques. Sampling and sample storage.

7. Occurence and significance of microorganisms in environment and their detection.

8. Requirements on microbiological laboratory. General instructions for microbiological handling.

9. Laboratory experiments. Safe handling in microbiological laboratory. Preparation of sterile materials.

10. Laboratory experiments. Control of hygiene and laboratory sanitation by both cultivation and rapid techniques.

11. Laboratory experiments. Enumeration of bacteria, yeasts and moulds in soil sample.

12. Laboratory experiments. Enumeration of both culturable and indicator microorganisms in water sample.

13. Laboratory experiments. Isolation techniques. Sample preparation for light microscopy.

14. Laboratory experiments. Testing the effect of external factors on the growth of microorganisms.

Last update: Chumchalová Jana (07.02.2018)
Registration requirements -

Basic knowledge from biology and microscopic techniques.

Last update: Chumchalová Jana (26.01.2018)
Course completion requirements -

Students must to undergo all laboratory tests, to write protocol under rules and to surrend it in term, to write a test and to obtain a minimum 50% of points and pass an oral examination.

Last update: Chumchalová Jana (26.01.2018)
Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1 28
Příprava na zkoušku a její absolvování 2 56
4 / 4 112 / 112
Coursework assessment
Form Significance
Report from individual projects 40
Examination test 30
Oral examination 30