Microbiology in Environmental Protection - M240014
Title: Mikrobiologie pro ochranu životního prostředí
Guaranteed by: Department of Environmental Chemistry (240)
Faculty: Faculty of Environmental Technology
Actual: from 2023
Semester: winter
Points: winter s.:5
E-Credits: winter s.:5
Examination process: winter s.:
Hours per week, examination: winter s.:2/2, C+Ex [HT]
Capacity: 8 / 8 (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
For type:  
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Chumchalová Jana Dr. Ing.
Interchangeability : N240023
Examination dates   
Annotation -
Last update: Chumchalová Jana Dr. Ing. (26.01.2018)
The course is directed to the theoretical and practical knowledge dealing with microorganisms in their environment. Lectures will include information on characterisation of microorganisms, degradation of substrates. Special attention will be paid to the influence of environmental conditions on metabolical and physiological activity, microorganism detection and enumeration. Requirements on microbiological laboratory and general instructions for safe microbiological handling will be mentioned in the last part. Part of the subject will be directed to the laboratory experiments. Students will be introduced into application of basic cultivation techniques and rapid methods for detection and characterization of microorganisms in environmental samples.
Aim of the course -
Last update: Chumchalová Jana Dr. Ing. (26.01.2018)

Students will be able to practically apply the knowledge from the field of:

  • microorganism taxonomy,
  • metabolism and genetic,
  • both theoretical and practical basic laboratory techniques used for determination and characterization of microorganisms.

Literature -
Last update: Chumchalová Jana Dr. Ing. (26.01.2018)

Pepper I.L., Gerba G.P., Gentry T.J. Environmental Microbiology. 3. vyd. Elsevier, 2015. ISBN 9780123946263

Learning resources -
Last update: Chumchalová Jana Dr. Ing. (07.02.2018)


Chumchalová J., Kubal M.: Praktická cvičení z environmentální mikrobiologie, VŠCHT Praha, 2017. https://uchop.vscht.cz/ke-stazeni/materialy

Miniatlas mikroorganismů. http://old.vscht.cz/obsah/fakulty/fpbt/ostatni/miniatlas/index.htm

Requirements to the exam -
Last update: Chumchalová Jana Dr. Ing. (26.01.2018)

Rules to grant credit: student will attend laboratories and deliver report from laboratory praxis in term.

Rules to get exam: student will consist within syllabus of a written part and then listener will be interviewed orally.

Syllabus -
Last update: Chumchalová Jana Dr. Ing. (07.02.2018)

1. Specification of the subject Microbiology in environment, basic microbiological terms, microbiological taxonomy.

2. Basic structure of microbial cells and their chemical content.

3. Metabolical processes in microorganism.

4. Influence of internal and external factors on growth and characteristics of microorganisms.

5. Nutrition of microorganisms. Growth curve.

6. Principles of both detection and enumeration of microorganisms by cultivation techniques. Sampling and sample storage.

7. Occurence and significance of microorganisms in environment and their detection.

8. Requirements on microbiological laboratory. General instructions for microbiological handling.

9. Laboratory experiments. Safe handling in microbiological laboratory. Preparation of sterile materials.

10. Laboratory experiments. Control of hygiene and laboratory sanitation by both cultivation and rapid techniques.

11. Laboratory experiments. Enumeration of bacteria, yeasts and moulds in soil sample.

12. Laboratory experiments. Enumeration of both culturable and indicator microorganisms in water sample.

13. Laboratory experiments. Isolation techniques. Sample preparation for light microscopy.

14. Laboratory experiments. Testing the effect of external factors on the growth of microorganisms.

Registration requirements -
Last update: Chumchalová Jana Dr. Ing. (26.01.2018)

Basic knowledge from biology and microscopic techniques.

Course completion requirements -
Last update: Chumchalová Jana Dr. Ing. (26.01.2018)

Students must to undergo all laboratory tests, to write protocol under rules and to surrend it in term, to write a test and to obtain a minimum 50% of points and pass an oral examination.

Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1 28
Příprava na zkoušku a její absolvování 2 56
4 / 5 112 / 140