Separation Methods in Biotechnology - M319006
Title: Separace v biotechnologiích
Guaranteed by: Department of Biotechnology (319)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:2/1, C+Ex [HT]
Capacity: unlimited / unlimited (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Guarantor: Melzoch Karel prof. Ing. CSc.
Interchangeability : N319009
Examination dates   
This subject contains the following additional online materials
Annotation -
The subject "Separation Methods in Biotechnology" expands students knowledge oriented towards engineering and technical aspects of biotechnological processes. Students will become familiar with separation processes as unit operations, their principles, roles and inclusion into biotechnologies.
Last update: Melzoch Karel (17.09.2023)
Aim of the course -

Students will be able to:

Define the structure of biotechnological process

Undersand the bioprocesses from engineering perspective

Describe the separation processes as a unit operation

Describe the function and role separation processes in biotechnology

Define the fundamental principles of separation processes

Integrate the separation processes into biotechnology

Select suitable separation process with regard to the requirements for quality and purity of the product

Choose the sequence of separation processes, particularly in the isolation, purification and finishing of the product

Optimize the separation processes in the up-stream and down-stream processing

Clasify separation techniques in terms of efficiency and economy

As part of the exercise, students will prepare and present a case study on a topic from the area of separation processes, for which they will receive credit.

Last update: Melzoch Karel (17.09.2023)
Literature -

R: Wang W. K. (2001): Membrane Separations in Biotechnology. 2. vyd., Marcel Dekker, Inc., New York, Basel. ISBN: 0-8247-0248-4

R: Fellows P. J. (2009): Food Processing Technology, Principles and Practice. 3. vyd., Woodhead Publishing Ltd, Cambridge a CRC Press, Boca Raton. ISBN: 978-1-84569-216-2

R: Harrison R. G., Todd P., Rudge S. R., Petrides D. P. (2003): Bioseparations Science and Engineering. Oxford University Press, New York,Oxford. ISBN: 0-19-512340-9.

A: Kadlec P., Melzoch K., Voldřich M. (2011): Procesy a zařízení potravinářských a biotechnologických výrob. KEY Publishing s.r.o., Ostrava. ISBN: 978-80-7418-086-6

A: Palatý Z. (2012): Membránové procesy. Vydavatelství VŠCHT Praha, ISBN: 978-80-7080-808-5

Last update: Melzoch Karel (17.09.2023)
Learning resources - (e-books available in the domain of ICT Prague)

Last update: Hladíková Jana (20.12.2017)
Syllabus -

1. Biotechnological process as sequence of unit operations - from substrate to product

2. Selection of useful separation process - properties of separated materials and bioproducts

3. Separation processes based on different particle size. Membrane processes, membranes, modules - basic characteristics

4. Filtration, microfiltration, ultrafiltration, nanofiltration, reverse osmosis

5. Membrane distillation, pervaporation, gas permeation

6. Electromigration processes - electrophoresis

7. Elektrodialysis, membrane electrolysis

8. Liquid membranes, pertraction, reactive membranes

9. Supercritical extraction. Two-phase water extraction. Extraction of biopolymers

10. Preparative chromatography - principles, stationary phases and arrangement. Adsorption chromatography. Molecular sieve chromatography

11. Ion-exchange chromatography

12. Hydrophobic interaction chromatography, reverse phase chromatography, affinity chromatography - biospecific interactions

13. Integrated processes. Membrane bioreactors. Industrial applications.

14. How to choose effective separation and purification sequence - strategy and economy

Last update: Melzoch Karel (17.09.2023)
Entry requirements -

Basic knowledge of physical chemistry, chemical engineering, biochemistry, microbiology, bioengineering, food and biochemical technologies, ...

Last update: Melzoch Karel (17.09.2023)
Registration requirements -

Microbiology, Biochemistry, Physical chemistry, Chemical engineering, Bioengineering, Food and Biochemical Technology

Last update: Melzoch Karel (17.09.2023)