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The purpose of this course is to acquire complex skills which enable to plan, prepare and carry out experiments and evaluate both on-line and off-line data gained in the process. The data are then used for matematical modelling of these processes.
Last update: Branská Barbora (25.04.2025)
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Participation in practical lessons Elaboration of reports from individual tasks Presentation of the final project Last update: Branská Barbora (25.04.2025)
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R: Moo-Young M.: Comprehensive biotechnology, Elsevier B.V., 2011, ISBN: 9780080885049 R: Scragg A.: Environmental Biotechnology, Oxford University Press, 2005, ISBN: 0-19-926867-3 R: Basařová G., Šavel J., Basař P., Lejsek T.: Pivovarství: Teorie a praxe výroby piva, VŠCHT Praha (2. vydání, 2021), ISBN: 978-80-87109-71-7 Last update: Branská Barbora (25.04.2025)
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1. Preparation of medium, inoculum, methods of storage of production cultures (freezing with glycerol, lyophilization, preservation on agar) 2. Cultivation on solid substrate, determination of growth 3. Use of molecular biology techniques in biotechnology - DNA isolation, PCR, electrophoresis on acrylamide gel 4. Expression, isolation and purification of recombinant proteins, product analysis 5. Determination of KLa, preparation of the fermenter for cultivation (assembly, sterilization, calibration of electrodes) 6. Malting of barley in a micro malting plant, control and monitoring of the process 7. Determination of basic technological parameters of malt, determination of carbohydrate content in malt 8. Brewing process, preparation of wort, fermentation, monitoring of the fermentation process, evaluation of the process 9. Analyzes of brewery-significant properties of raw materials and basic product parameters 10. Monitoring the formation of bacterial biofilm and the influence of active substances on its adhesion and eradication 11. Preparation and application of a biostimulating preparation, evaluation of the effect on plants using a 3D scanner and a multispectral camera 12. Characteristics of the flow of phases in the reactor - distribution of delay times, ideal flow, piston flow, non-ideal flow 13. Work on an individual project 14. Presentation of the project Last update: Branská Barbora (25.04.2025)
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Students will be able to carry out: Long term storage of strains, inoculum preparation Experiments planning, cultivation in shaken flasks and bioreactors, production of primary and/or secondary metabolites Analytical procedures used in biotechnology Data evaluation Modelling of bioprocesses Last update: Branská Barbora (25.04.2025)
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Bioengineering, Microbiology Last update: Branská Barbora (14.05.2025)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Konzultace s vyučujícími | 0.7 | 20 | ||
Účast v laboratořích (na exkurzi nebo praxi) | 2 | 56 | ||
Obhajoba individuálního projektu | 0.2 | 6 | ||
Účast na přednáškách | 0.5 | 14 | ||
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 1.3 | 36 | ||
Práce na individuálním projektu | 1.3 | 36 | ||
Účast na seminářích | 1 | 28 | ||
7 / 7 | 196 / 196 |