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The course is focused on solving practical problems in the biochemical laboratory. The course summarizes the findings of analytical chemistry, biochemistry and biophysical chemistry.
Last update: Kučková Štěpánka (11.01.2018)
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Successful completion of the written examination. Last update: Kučková Štěpánka (11.01.2018)
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R: Kodíček M., Karpenko V., Biofyzikální chemie, Academia, 2000, 80-200-0791-1 R: Káš J., Kodíček M., Valentová O., Laboratorní techniky biochemie, Vysoká škola chemicko-technologická v Praze, 2006, 80-7080-586-2 A: Churáček J. a kol., Analytická separace látek, SNTL, Praha 1990, 80-03-00569-8 Last update: Kučková Štěpánka (11.01.2018)
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1. Basic terms (measurements, quantities, characteristics of analytical methods), repetition of analytical calculations 2. Electroanalytical methods, preparation of distilled water, optical methods in biochemical laboratory 3. Biosensors, potentiometric sensors, the principles of pH measurements and maintenance of electrodes 4. Optical, thermal and piezoelectric transducers, sensors based on sensitive polymer layer, requirements for ideal biosensor 5. Procedures used for sample preparation for analysis, filtration, ultrafiltration, dialysis, sonication, microwave devices 6. Robotics, automation, system miniaturization 7. Separation methods, flow cytometry, centrifuges and centrifugal methods, rotors, field flow fractionation 8. Electrophoretic methods I: basic terms, separation modes 9. Electrophoretic methods II: electrophoretic titration curve, detection, blotting, processing and storage of electropherograms 10. Separation methods I: basic terms, methods, Van Deemter equation, TLC methods 11. Separation methods II: liquid chromatography, diagnostic of problems with chromatographic separation, gel chromatography 12. Detectors used in chromatography, derivatization of analytes, maintenance rules, selection of appropriate equipment 13. Vibrational spectroscopic methods - FTIR, Raman spectroscopy, immuno-SERS 14. Multidimensional separation techniques in proteomics, combining of different separation techniques and detectors
Last update: Kučková Štěpánka (11.01.2018)
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None Last update: Kučková Štěpánka (11.01.2018)
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Students will be able: To solve practical problems that commonly occurring in a biochemical laboratory. Last update: Kučková Štěpánka (11.01.2018)
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Analytical Chemistry, Biochemistry, Biophysical Chemistry Last update: Kučková Štěpánka (11.01.2018)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Účast na přednáškách | 1 | 28 | ||
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 0.5 | 14 | ||
Příprava na zkoušku a její absolvování | 1.5 | 42 | ||
3 / 3 | 84 / 84 |