SubjectsSubjects(version: 955)
Course, academic year 2019/2020
Advanced Biochemistry: Laboratory - M320012
Title: Laboratoř speciální biochemie
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2019 to 2019
Semester: winter
Points: winter s.:3
E-Credits: winter s.:3
Examination process: winter s.:
Hours per week, examination: winter s.:0/5, MC [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Guarantor: Lipov Jan doc. Ing. Ph.D.
Novotná Zuzana doc. Dr. Ing.
Class: Základní laboratoře
Specializační laboratoř
Interchangeability : N320036
Examination dates   Schedule   
Annotation -
Tha aim of the subject is to expand the basic knowledge of the biochemical course and to adopt new techniques of modern biochemistry and to amend the spectrum of techniques of biochemistry and molecular biology used in research laboratories.
Last update: Hladíková Jana (13.12.2017)
Aim of the course -

Students will be able to:

isolate the proteins from various sources

characterize the enzyme activity

determine the degree of cooperativity of the enzyme during its denaturation

identify the protein by mass spectrometry

isolate plasmid DNA and characterize it by restriction cleavage

analyze and process the results to protocols

Last update: Hladíková Jana (13.12.2017)
Literature -

R: Káš J., Kodíček M., Valentová O.: Laboratorní techniky biochemie. 1. vyd. Vysoká škola chemicko-technologická v Praze, Praha 2006. ISBN 80-7080-586-2

Last update: Hladíková Jana (13.12.2017)
Learning resources -

Kodíček Milan: Biochemické pojmy : výkladový slovník (

Last update: Hladíková Jana (13.12.2017)
Requirements to the exam -

Elaboration of protocols from all laboratory tasks.

Classification at least E from every protocol.

At least 50% from the final written test.

Last update: Lipov Jan (25.01.2018)
Syllabus -

1. Good laboratory practice, safety

2. Theoretical introduction to the laboratory tasks

3. Chromatography of biomacromolecules, evaluation of purification steps

4. Electrophoretic analysis of erythrocyte membrane proteins

5. Basic characterization of purified preparates of proteins

6. Enzyme kinetics (allosteric enzymes)

7. Modern methods of protein identification

8. Physical & chemical properties of proteins

9. Immunotechniques

10.Basic techniques of molecular biology

11.Isolation of subcellular particles

12.Study of basic metabolic events

13.Methods of determination of catalytical activity of enzymes

14.Evaluation, test and free discussion

Last update: Hladíková Jana (13.12.2017)
Registration requirements -


Laboratory of biochemistry

Last update: Lipov Jan (27.02.2018)
Course completion requirements -

Credit will be obtained based on a written solution of an individual task in given deadline. The exam comprises a written part.

Last update: Novotná Zuzana (25.01.2018)
Teaching methods
Activity Credits Hours
Účast v laboratořích (na exkurzi nebo praxi) 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1 28
Příprava na zkoušku a její absolvování 1 28
3 / 3 84 / 84
Coursework assessment
Form Significance
Report from individual projects 40
Continuous assessment of study performance and course -credit tests 60