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Last update: Hladíková Jana (13.12.2017)
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Last update: Hladíková Jana (13.12.2017)
Students will be able to: isolate the proteins from various sources characterize the enzyme activity determine the degree of cooperativity of the enzyme during its denaturation identify the protein by mass spectrometry isolate plasmid DNA and characterize it by restriction cleavage analyze and process the results to protocols |
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Last update: Hladíková Jana (13.12.2017)
R: Káš J., Kodíček M., Valentová O.: Laboratorní techniky biochemie. 1. vyd. Vysoká škola chemicko-technologická v Praze, Praha 2006. ISBN 80-7080-586-2 |
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Last update: Hladíková Jana (13.12.2017)
Kodíček Milan: Biochemické pojmy : výkladový slovník (http://vydavatelstvi.vscht.cz/knihy/uid_es-002/) |
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Last update: Lipov Jan doc. Ing. Ph.D. (25.01.2018)
Elaboration of protocols from all laboratory tasks. Classification at least E from every protocol. At least 50% from the final written test. |
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Last update: Hladíková Jana (13.12.2017)
1. Good laboratory practice, safety 2. Theoretical introduction to the laboratory tasks 3. Chromatography of biomacromolecules, evaluation of purification steps 4. Electrophoretic analysis of erythrocyte membrane proteins 5. Basic characterization of purified preparates of proteins 6. Enzyme kinetics (allosteric enzymes) 7. Modern methods of protein identification 8. Physical & chemical properties of proteins 9. Immunotechniques 10.Basic techniques of molecular biology 11.Isolation of subcellular particles 12.Study of basic metabolic events 13.Methods of determination of catalytical activity of enzymes 14.Evaluation, test and free discussion |
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Last update: Lipov Jan doc. Ing. Ph.D. (27.02.2018)
Biochemistry Laboratory of biochemistry |
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Last update: Novotná Zuzana doc. Dr. Ing. (25.01.2018)
Credit will be obtained based on a written solution of an individual task in given deadline. The exam comprises a written part. |
Teaching methods | ||||
Activity | Credits | Hours | ||
Účast v laboratořích (na exkurzi nebo praxi) | 1 | 28 | ||
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 1 | 28 | ||
Příprava na zkoušku a její absolvování | 1 | 28 | ||
3 / 3 | 84 / 84 |