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The subject is focused on understanding of principles of manipulation with nucleic acids and their analysis. It covers information about various methods of introducing genes into diverse cell types, determination of gene expression and analysis. The goal is to deliver information about genetic engineering techniques in order to facilitate the choce of the optimal method for a particular application. The lectures cover basic methods of isolation, analysis and modification of nucleic acids and special applications as gene modification for detection or affinity purification of gene product, study of interaction of proteins and nucleic acids, or gene therapies.
Last update: Hladíková Jana (13.12.2017)
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Students will be able to: Isolate DNA and perform basic manipulations with recombinant DNA. Prepare vectors for genetic modification of various cell types. Modify and label DNA, apply DNA labeled probes for identification of specific sequences. Modify genes by directed mutagenesis by directed mutagenesis, extension by sequences for detection and purification by affinity chromatography. Analyze production of heterologous proteins and isolate them from various cell types. Analyze protein interactions. Construct gene libraries. Last update: Hladíková Jana (13.12.2017)
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Passing written test with a score exceeding 50% and passing an oral exam Last update: Ruml Tomáš (26.01.2018)
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Sue Carson, Heather B. Miller, Melissa C. Srougi, D. Scott Witherow: Molecular Biology Techniques A Classroom Laboratory Manual 4th Edition, Elsevier 2019 - D D:Green M. R., Sambrook J., Molecular Cloning: A Laboratory Manual (Fourth Edition); Cold Spring Harbor Laboratory Press 2012 - D Last update: Ruml Tomáš (15.09.2023)
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Předmět je zakončen písemnou a ústní zkouškou. Last update: Lipovová Petra (09.08.2023)
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1. Recapitulation of genetic principles. 2. Plasmid and viral vectors, artificial chromosomes. 3. DNA production and isolation, its analysis. 4. Restriction endonucleases, genome mapping, 5. Modification of nucleic acids for detection of specific sequences. 6. High-capacity methods of DNA sequencing. 7. Principles of work with RNA, subtraction libraries. 8. Application of polymerase chain reaction: product modification, controlled mutagenesis. 9. Protein production in microbial cells, fusion proteins, reporter genes. 10. Tissue cultures and their use for gene expression. 11. Principle of transgenic organisms preparation. 12. Detection of gene expression products - metabolic labeling, electrophoretic and immunochemical methods. 13. Gene therapy. 14. Methods for studying protein-nucleic acid interactions. Last update: Ruml Tomáš (13.09.2019)
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E-learning VŠCHT Praha - slide presentations E-learning VŠCHT Praha - lectures Last update: Ruml Tomáš (15.09.2023)
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Biochemistry Molecular genetics or Molecular genetics and DNA analysis Last update: Kubová Petra (03.03.2018)
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| Teaching methods | ||||
| Activity | Credits | Hours | ||
| Účast na přednáškách | 1 | 28 | ||
| Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 0.4 | 12 | ||
| Příprava na zkoušku a její absolvování | 1.3 | 35 | ||
| 3 / 3 | 75 / 84 | |||