Genetic Engineering - M320017
Title: Genové inženýrství
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2020 to 2022
Semester: both
Points: 3
E-Credits: 3
Examination process:
Hours per week, examination: 2/0, Ex [HT]
Capacity: winter:unlimited / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course can be enrolled in outside the study plan
enabled for web enrollment
you can enroll for the course in winter and in summer semester
Guarantor: Ruml Tomáš prof. Ing. CSc.
Jablonská Eva Ing. Ph.D.
Interchangeability : AM320017, N320051
Is interchangeable with: AM320017
Examination dates   
This subject contains the following additional online materials
Annotation -
The subject is focused on understanding of principles of manipulation with nucleic acids and their analysis. It covers information about various methods of introducing genes into diverse cell types, determination of gene expression and analysis. The goal is to deliver information about genetic engineering techniques in order to facilitate the choce of the optimal method for a particular application. The lectures cover basic methods of isolation, analysis and modification of nucleic acids and special applications as gene modification for detection or affinity purification of gene product, study of interaction of proteins and nucleic acids, or gene therapies.
Last update: Hladíková Jana (13.12.2017)
Aim of the course -

Students will be able to:

Isolate DNA and perform basic manipulations with recombinant DNA. Prepare vectors for genetic modification of various cell types.

Modify and label DNA, apply DNA labeled probes for identification of specific sequences.

Modify genes by directed mutagenesis by directed mutagenesis, extension by sequences for detection and purification by affinity chromatography.

Analyze production of heterologous proteins and isolate them from various cell types.

Analyze protein interactions. Construct gene libraries.

Last update: Hladíková Jana (13.12.2017)
Literature -

Sue Carson, Heather B. Miller, Melissa C. Srougi, D. Scott Witherow: Molecular Biology Techniques A Classroom Laboratory Manual 4th Edition, Elsevier 2019 - D

D:Green M. R., Sambrook J., Molecular Cloning: A Laboratory Manual (Fourth Edition); Cold Spring Harbor Laboratory Press 2012 - D

Last update: Ruml Tomáš (15.09.2023)
Learning resources -

E-learning VŠCHT Praha - slide presentations

E-learning VŠCHT Praha - lectures

Last update: Ruml Tomáš (15.09.2023)
Syllabus -

1. Recapitulation of genetic principles.

2. Plasmid and viral vectors, artificial chromosomes.

3. DNA production and isolation, its analysis.

4. Restriction endonucleases, genome mapping,

5. Modification of nucleic acids for detection of specific sequences.

6. High-capacity methods of DNA sequencing.

7. Principles of work with RNA, subtraction libraries.

8. Application of polymerase chain reaction: product modification, controlled mutagenesis.

9. Protein production in microbial cells, fusion proteins, reporter genes.

10. Tissue cultures and their use for gene expression.

11. Principle of transgenic organisms preparation.

12. Detection of gene expression products - metabolic labeling, electrophoretic and immunochemical methods.

13. Gene therapy.

14. Methods for studying protein-nucleic acid interactions.

Last update: Ruml Tomáš (13.09.2019)
Registration requirements -


Molecular genetics or Molecular genetics and DNA analysis

Last update: Kubová Petra (03.03.2018)
Course completion requirements

Passing written test with a score exceeding 50% and passing an oral exam

Last update: Ruml Tomáš (26.01.2018)
Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 0.4 12
Příprava na zkoušku a její absolvování 1.3 35
3 / 3 75 / 84