SubjectsSubjects(version: 855)
Course, academic year 2019/2020
  
Biochemistry of Microorganisms - M320019
Title: Biochemie mikroorganismů
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2019
Semester: summer
Points: summer s.:3
E-Credits: summer s.:3
Examination process: summer s.:
Hours per week, examination: summer s.:2/0 Ex [hours/week]
Capacity: unlimited / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
Level:  
For type: Master's (post-Bachelor)
Guarantor: Stiborová Hana Ing. Ph.D.
Interchangeability : N320053
Examination dates   Schedule   
Annotation -
Last update: Hladíková Jana (13.12.2017)
The subject widens the basic knowledge of biochemistry of microorganisms targeted on the organization of cell metabolism and their regulatory mechanisms. Cell cycle regulation of prokaryotes and eukaryotes is part of this. Biochemical principles of extremophilic microorganisms are included.Next topics are determinants of pathogens virulence, synthesis of antimicrobials peptides and antibiotics (secondary metabolites). The quorum-sensing regulation and the formation of bacterial biofilm are explained.
Aim of the course -
Last update: Stiborová Hana Ing. Ph.D. (06.02.2018)

Student will gain the knowledge in these fields:

  • metabolism of different groups of microorganisms
  • regulation of processes as sporogenesis, cell cycle, secretion and transport of low and high molecular matters from the cells
  • mechanisms of action of antimicrobials agents
  • mechanisms effecting the microbial pathogenicity
  • responses of microorganisms to various types of stress (heat-shock, oxidative stress), VBNC cells
  • adaptation of microorganisms to extreme conditions.

Literature -
Last update: Stiborová Hana Ing. Ph.D. (09.02.2018)

Brock Biology of Microorganisms (15th Edition), Michael T. Madigan,‎ Kelly S. Bender ,‎ Daniel H. Buckley,‎ W. Matthew Sattley,‎ David A. Stahl, (2018), ISBN-13: 978-0321649638

Microbial Biochemistry (4th Edition), Georges N. Cohen, (ed. 2016), ISBN-10: 940177577X

Bacterial Physiology and Metabolism, Byung Hong Kim, Geoffrey Michael Gadd, Cambridge University Press; 1 edition (2008), ISBN-10: 0521712300

Learning resources -
Last update: Hladíková Jana (13.12.2017)

none

Syllabus -
Last update: Kubová Petra Ing. (14.12.2018)

1. Introduction. Cell metabolism organization.

2. DNA replication and repair, transcription and regulation, translation

3. Life and cell cyclus of prokaryotic and eukaryotic microorganisms, cytoskeleton.

4. Bioenergetics of microorganisms.

5. Biochemistry and adaptation mechanisms of extremophiles.

6. Synthesis of essentials amino acids.

7. Synthesis of secondary metabolites, antibiotic`s mechanism of action.

8. Inhibitory factors produced by microorganisms - antibiotics, colicines, killer factors.

9. Factors determining microorganisms patogenity.

10. Secretion system of prokaryotic and eukaryotic microorganisms.

11. Transport mechanisms in microorganisms.

12. Life cycles of microorganisms: biochemistry, regulation, and communication „Quorum sensing“.

13. Oxidative stress of microorganisms.

14. Microbial enzymes in biotechnology.

Registration requirements -
Last update: Hladíková Jana (13.12.2017)

Biochemistry, Mikrobiology

Course completion requirements -
Last update: Stiborová Hana Ing. Ph.D. (06.02.2018)

Only the student, who previously presented and defended the individual project on selected topic, can write final exam test and pass the oral exam. The successful completion of the course consists of the written test (min. 50 points out of 100) and oral examination in A-E grade.

Teaching methods
Activity Credits Hours
Obhajoba individuálního projektu 0,3 8
Účast na přednáškách 1 28
Příprava na zkoušku a její absolvování 1,7 48
3 / 3 84 / 84
Coursework assessment
Form Significance
Regular attendance 15
Defense of an individual project 20
Examination test 30
Oral examination 35

 
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