Synthetic biology and genetically modified organisms - M320020
Title: Syntetická biologie a geneticky modifikované organismy
Guaranteed by: Department of Biochemistry and Microbiology (320)
Actual: from 2020
Semester: summer
Points: summer s.:4
E-Credits: summer s.:4
Examination process: summer s.:
Hours per week, examination: summer s.:3/0 Ex [hours/week]
Capacity: unlimited / unknown (unknown)
Min. number of students: unlimited
Language: Czech
Teaching methods: full-time
For type: Master's (post-Bachelor)
Guarantor: Zdeňková Kamila Ing. Ph.D.
Viktorová Jitka Ing. Ph.D.
Interchangeability : N320062
Examination dates   
This subject contains the following additional online materials
Annotation -
Last update: Zdeňková Kamila Ing. Ph.D. (23.01.2018)
The subject is focused on organisms modified by genetic methods in order to deepen the knowledge in the field of genetic engineering and possibilities of its utilization. Individual methods of preparation, utilization and detection of modified microorganisms, plants and animals are studied. Attention is also paid to new breeding techniques including epigenetic changes. Examples of the use of genomic editing methods and extensive organisms (SynBio) have also been explored. The course also includes legislation on GMO management, database work, use ethics, and potential risk assessment of modified organisms.
Aim of the course -
Last update: Zdeňková Kamila Ing. Ph.D. (23.01.2018)

Students will be able to:

• Understand the possibilities of using genetically modified organisms;

• Design a strategy for the preparation of a modified organism;

• Detect GMOs or GMO products;

• Know the specific possibilities of using synthetic biology;

• Work with DNA sequences in Internet databases and programs.

Literature -
Last update: Zdeňková Kamila Ing. Ph.D. (23.01.2018)

Original and overall articles in scientific and technical journals, patent literature, monographs and electronic books.

Primrose S.B., Twyman R.M., Old R. W.: Principles of Gene Manipulation and Genomics, Blackwell Publishing, 2006. ISBN-13: 978-1405135443.

Altman A., Haseqawa P. M.: Plant Biotechnology and Agriculture: Prospects for the 21st Century 1st Edition,Elsevier, 2012. ISBN-13: 978-0123814661

Slater A., Scott W., Fowler M.R., Plant Biotechnology, Oxford, 9780199282616

Zhao, Huimin: Synthetic biology: tools and applications. Edited by Huimin Zhao. Amsterdam: Elsevier, 2013, 333. ISBN 9780123944306.

Schmidt M.: Synthetic biology: industrial and environmental applications. Weinheim: Wiley-Blackwell, 2012. 240. ISBN 9783527331833.

Learning resources -
Last update: Zdeňková Kamila Ing. Ph.D. (23.01.2018)

Syllabus -
Last update: Zdeňková Kamila Ing. Ph.D. (23.01.2018)

1. Importance of genetically modified organisms, basic concepts. Current legislation and labelling of products like "GMO free".

2. Strategy for the preparation of genetically modified organisms.

3. Preparation of genetically modified micro-organisms.

4. Use of genetically modified micro-organisms.

5. Preparation of genetically modified plants.

6. Use of genetically modified plants.

7. Preparation and use of genetically modified animals.

8. New breeding techniques (NBT), nucleases recognizing the specific nucleic acid sequence ZFN, TALEN and CRISPR. Correction of DNA breaks – non-homological linking of ends and homologous recombination. Examples of organisms prepared via NBT and their use.

9. Plant genome edit, edit animal populations (gene drive), detection of editation.

10. Epigenetics, methods of epigenetic phenomena study, technique of RNA-dependent DNA methylation (RdDM).

11. Methods of determination and quantification of transgenic organisms.

12. Definition and brief overview of the history of the field of Synthetic Biology (SynBio). Minimal genome and transplantation of the synthetic genome of the bacterium.

13. Follow-up examples of SynBio uses: Definition and brief overview of the history of the field of Synthetic Biology (SynBio). Examples of SynBio use: minimal genome; transplantation of the synthetic genome of the bacterium; Xeno nucleic acid (XNA), DNA bricks, BioBrick (iGEM competition), metabolic engineering, and more.

14. Bioinformatics, database, primer design. Ethics of use and potential risks of modified organisms (bio-security and bio-protection).

Registration requirements -
Last update: Hladíková Jana (13.12.2017)

Molecular genetics

Course completion requirements -
Last update: Zdeňková Kamila Ing. Ph.D. (26.01.2018)

Seminar work, credit test and written exam.

Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Práce na individuálním projektu 1 28
Příprava na zkoušku a její absolvování 2 56
4 / 4 112 / 112