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Last update: Hrabal Richard prof. Ing. CSc. (24.01.2018)
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Last update: Hrabal Richard prof. Ing. CSc. (24.01.2018)
Students will be able to: They will get an overview of modern analytical techniques common nowadays in biological sciences. They will learn the fundaments of each method and possible applications in life sciences. They will also be able to process and use experimental data from each instrumental method in their own work. |
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Last update: Spiwok Vojtěch prof. Ing. Ph.D. (10.08.2023)
Z: https://clab.vscht.cz/nmr/vyuka/struktbio D: Renaud J.-P.: Structural Biology in Drug Discovery, 2020, John Wiley & Sons, Inc. ISBN: 9781118681015 (available via NTK) D: Spence J.C.H. High-resolution electron microscopy. 2013, 4th. ed. Oxford University Press, ISBN: 0199668639 (available via NTK) |
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Last update: Spiwok Vojtěch prof. Ing. Ph.D. (09.08.2023)
Computing methods: http://web.vscht.cz/spiwokv/modelovani/ NMR: http://en.wikipedia.org/wiki/Nuclear_magnetic_resonance |
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Last update: Cibulková Jana Ing. (13.09.2023)
Výuka je zakončena klasifikovaným zápočtem formou písemného testu. Konečné hodnocení je možné vylepšit ústním pohovorem. |
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Last update: Hrabal Richard prof. Ing. CSc. (19.01.2018)
1. Structural databases, basic operations with biomolecular structures, molecular electrostatics 2. Prediction of protein structures, docking, virtual screening 3. Molecular mechanics, QM/MM, molecular dynamics, protein folding 4. Bases of nuclear magnetic resonance spectroscopy, (NMR), chemical shift, coupling constant, instrumental equipment 5. Technology of structure determination of proteins and their complexes, study of dynamical behavior. Practical examples of using NMR 6. Introduction to X-ray crystallography, crystal preparation 7. Diffraction theory, collection of diffraction data, phase problem, structure determination. Model construction, structure validation 8. Principles and basic techniques of electron microscopy, TEM, SEM and their using in biological sciences 9. Atomic force microscopy AFM, scanning probe microscopy SPM, scanning tunneling microscopy STM 10. Introduction to protein mass spectrometry, ionizations techniques, detectors, fragmentation techniques 11. Applications of MS in biological sciences (molecular mass determination, protein identification, sequential analysis…. Practical examples of using MS) 12. Optical microscopy 13. Superresolution microscopy 14. Surface plasmon resonance |
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Last update: Ulbrich Pavel doc. Ing. Ph.D. (23.01.2018)
Students should have basic knowledge of mathematics, physics and chemistry, especially biochemistry and instrumental analysis. |
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Last update: Hrabal Richard prof. Ing. CSc. (26.01.2018)
To pass the exam the students have to complete successfuly a written test. There is an option to improve the final grade by an oral exam. |
Teaching methods | ||||
Activity | Credits | Hours | ||
Konzultace s vyučujícími | 0.2 | 5 | ||
Účast na přednáškách | 1.5 | 42 | ||
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi | 0.2 | 5 | ||
Příprava na zkoušku a její absolvování | 1.1 | 30 | ||
3 / 3 | 82 / 84 |