SubjectsSubjects(version: 965)
Course, academic year 2024/2025
  
Proteomic Analysis - M320084
Title: Proteomická analýza
Guaranteed by: Department of Biochemistry and Microbiology (320)
Faculty: Faculty of Food and Biochemical Technology
Actual: from 2023
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:1/2, C+Ex [HT]
Capacity: unlimited / unlimited (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Level:  
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Šantrůček Jiří Ing. Ph.D.
Annotation -
The subject is focused on proteomics as a modern way of study of complex biological systems. The subject goal is to guide students from the design of an experiment through acquisition of the proteomic data to biological interpretation. Lectures on the structural proteomics and application lectures from fields where the proteomics is used are also included.
Last update: Šantrůček Jiří (19.06.2023)
Literature -

Gross J.H., Mass spectrometry, Springer International Publishing, 2017, ISBN 978-3-319-54397-0

Pazos F., Chagoyen M., Practical protein bioinformatics, Springer International Publishing, 2015, ISBN 978-3-319-12726-2

Bessant C. (editor), Proteome Informatics, The Royal Society of Chemistry, 2017, ISBN 978-1-78262-428-8, https://doi.org/10.1039/9781782626732

Last update: Šantrůček Jiří (19.06.2023)
Syllabus -

1. Introduction to proteomic analysis - overview, sample preparation

2. Separation methods in proteomics

3. Mass spectrometry - instrumentation, tandem mass spectrometry

4. Identification of proteins I - peptide mass fingerprinting, identification using MS/MS data, de novo sequencing

5. Identification of proteins II - spectral libraries, identification of proteins from DIA runs

6. Quantification of proteins I - overview, label-based methods

7. Quantification of proteins II - label-free methods, targeted quantification

8. Analysis of posttranslational modifications

9. Biological interpretation of proteomic data

10. Proteomics vs. other -omics sciences

11. Structural proteomics - amino acid modification, crosslinking, hydrogen/deuterium exchange

12. Non-mass spectrometry based proteomic methods

13. Applied proteomics I - identification of microorganisms and other medical applications

14. Applied proteomics II - mass spectrometry imaging, food and forensic applications of proteomics

Last update: Šantrůček Jiří (19.06.2023)
Learning resources -

Lectures are available (in czech language) at e-learning.vscht.cz

Last update: Šantrůček Jiří (19.06.2023)
Learning outcomes -

Students will be able to:

  • design a proteomic experiment
  • identify and quantify proteins using mass spectrometric data
  • mine the acquired data for biological knowledge
Last update: Šantrůček Jiří (19.06.2023)
Registration requirements -

Biochemistry I

Analytical Chemistry I

Last update: Šantrůček Jiří (19.06.2023)
Teaching methods
Activity Credits Hours
Účast na přednáškách 1 28
Příprava na zkoušku a její absolvování 1.5 42
Účast na seminářích 1.5 42
4 / 4 112 / 112
 
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