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The subject is focused on proteomics as a modern way of study of complex biological systems. The subject goal is to guide students from the design of an experiment through acquisition of the proteomic data to biological interpretation. Lectures on the structural proteomics and application lectures from fields where the proteomics is used are also included.
Last update: Šantrůček Jiří (19.06.2023)
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Gross J.H., Mass spectrometry, Springer International Publishing, 2017, ISBN 978-3-319-54397-0
Pazos F., Chagoyen M., Practical protein bioinformatics, Springer International Publishing, 2015, ISBN 978-3-319-12726-2
Bessant C. (editor), Proteome Informatics, The Royal Society of Chemistry, 2017, ISBN 978-1-78262-428-8, https://doi.org/10.1039/9781782626732 Last update: Šantrůček Jiří (19.06.2023)
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1. Introduction to proteomic analysis - overview, sample preparation 2. Separation methods in proteomics 3. Mass spectrometry - instrumentation, tandem mass spectrometry 4. Identification of proteins I - peptide mass fingerprinting, identification using MS/MS data, de novo sequencing 5. Identification of proteins II - spectral libraries, identification of proteins from DIA runs 6. Quantification of proteins I - overview, label-based methods 7. Quantification of proteins II - label-free methods, targeted quantification 8. Analysis of posttranslational modifications 9. Biological interpretation of proteomic data 10. Proteomics vs. other -omics sciences 11. Structural proteomics - amino acid modification, crosslinking, hydrogen/deuterium exchange 12. Non-mass spectrometry based proteomic methods 13. Applied proteomics I - identification of microorganisms and other medical applications 14. Applied proteomics II - mass spectrometry imaging, food and forensic applications of proteomics Last update: Šantrůček Jiří (19.06.2023)
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Lectures are available (in czech language) at e-learning.vscht.cz Last update: Šantrůček Jiří (19.06.2023)
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Students will be able to:
Last update: Šantrůček Jiří (19.06.2023)
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Biochemistry I Analytical Chemistry I Last update: Šantrůček Jiří (19.06.2023)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Účast na přednáškách | 1 | 28 | ||
Příprava na zkoušku a její absolvování | 1.5 | 42 | ||
Účast na seminářích | 1.5 | 42 | ||
4 / 4 | 112 / 112 |