Subjects

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Chemistry of Natural Compounds: Laboratory I - M342015

Title:	Laboratoř Chemie přírodních látek I
Guaranteed by:	Department of Chemistry of Natural Compounds (342)
Faculty:	Faculty of Food and Biochemical Technology
Actual:	from 2023
Semester:	summer
Points:	summer s.:7
E-Credits:	summer s.:7
Examination process:	summer s.:
Hours per week, examination:	summer s.:0/10, MC [HT]
Capacity:	unknown / unknown (unknown)
Min. number of students:	unlimited
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Teaching methods:	full-time
Level:
Belongs to the student work of type:	projekt
Note:	course can be enrolled in outside the study plan enabled for web enrollment

Guarantor:	Raich Ivan doc. Dr. Ing.
Class:	Specializační laboratoř

Examination dates

Annotation -

Lab training is designed for works whose result would be few tens of milligrams (ca 50 mg) of pure compound which is a sufficient amount for chemical characterization. The intention is to learn to work with small amounts and with special equipment and labware as is usual for research work isolating compounds of natural origin.

Last update: Fialová Jana (20.12.2017)

Aim of the course -

Students will be able to:

Isolate and purify small amounts of compounds from natural materials

Work with labware designed for semi-micro preparative scale

Design procedures and equipment suitable for work with small amounts of compounds

Perform spectral and other characterization from small amounts of isolated compounds

Last update: Fialová Jana (20.12.2017)

Course completion requirements - Czech

Odevzdání preparátů a/nebo postupů vedoucímu práce.

Zpracování experimentální činnosti do podoby písemného odborného sdělení a odevzdání vedoucímu práce.

Last update: MORAVCOJ (27.02.2018)

Literature -

R: Mayo D. W., Pike R. M., Butcher S. S.: Microscale Oganic Laboratory , John Wiley and Sons, Inc., 2nd ed., New York, 1989

Last update: Fialová Jana (20.12.2017)

Syllabus -

1. Labor safety in laboratory, construction of apparatuses for basic operations

2. Isolation of caffeine from tea, extraction, sublimation, melting point

3. Isolation of trehalose from yeast, deproteinization, HPLC verification of product purity

4. Isolation of piperine from black pepper, extraction, decantation, TLC, crystallization

5. Isolation of botulin from birch bark, extraction, crystallization, derivatization

6. Isolation of cinnamaldehyde from cinnamon using steam distillation

7. Isolation of D-glucosamine from crab shells, decalcification, hydrolysis, crystallization

8. GLC analysis of cinnamaldehyde

9. HPLC analysis of trehalose on ion-exchange resin

10. NMR spectra measurements of prepared samples

11. IR spectra measurements of prepared samples

Last update: Fialová Jana (20.12.2017)

Learning resources -

Raich I., Medonos M.: Instructions for Isolation and separation methods: Laboratory, ICT Prague, 2012

Last update: Fialová Jana (20.12.2017)

Registration requirements -

Organic chemistry I

Organic chemistry: Laboratory I

Last update: Fialová Jana (20.12.2017)