Separation Methods - M402002
Title: Pokročilé separační metody
Guaranteed by: Department of Analytical Chemistry (402)
Faculty: Faculty of Chemical Engineering
Actual: from 2019 to 2019
Semester: winter
Points: winter s.:4
E-Credits: winter s.:4
Examination process: winter s.:
Hours per week, examination: winter s.:2/1, C+Ex [HT]
Extent per academic year: 2 [hours]
Capacity: unlimited / unlimited (unknown)
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Additional information:
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Sýkora David prof. Dr. RNDr.
Řezanka Pavel doc. RNDr. Ing. Ph.D.
Is co-requisite for: M402042
Examination dates   Schedule   
This subject contains the following additional online materials
Annotation -
The lectures are focused on separation techniques. Besides well known techniques less common methods that have great separation potencial are also discussed.
Last update: Řezanka Pavel (31.01.2018)
Aim of the course -

Students will be able to:

be well informed about separation methods

solve problems of real samples separations

evaluate and interpret data obtained by separation analysis.

Last update: Pátková Vlasta (05.01.2018)
Literature -

R: Analytická separace látek - kniha, J. Churáček; SNTL, 1990.

R: High Performance Capillary Electrophoresis - firemní příručka, D.N. Heiger, Hewlett-Packard, 1992.

R: Basic Gas Chromatography - kniha, H.M. McNair and J.M. Miller, Wiley, 2009, ISBN: 978-0-470-43954-8.

R: Practical High-Performance Liquid Chromatography - kniha, V.R. Meyer, Wiley, 2010, ISBN:978-0-470-68218-0.

R: Introduction to Mass Spectrometry - kniha, J.T. Watson and O.D. Sparkman, Wiley, 2007, ISBN: 978-0-470-51634-8.

A: Mass Spectrometry-A Textbook - kniha, J.H. Gross, 2004, Springer, ISBN: 3-540-40739-1.

A: Introduction to Modern Liquid Chromatography - kniha, L.R. Snyder, J.J. Kirkland and J.W. Dolan, Wiley, 2010, ISBN:978-0-470-16754-0.

A: Gas Chromatography and Mass Spectrometry-A Practical Guide - kniha, O.D. Sparkman, Z.E. Penton, and F.G. Kitson, 2011, Elsevier, ISBN:978-0-12-373628-4.

A: Practical HPLC Method Development - kniha, L.R. Snyder, J.J. Kirkland, J.L. Glajch; Wiley, 1997, ISBN:0-471-00703-X.

A: Chromatographic methods - kniha, A. Braithwaite, F.J. Smith; Blackie Academic & Proffessional, 1996, ISBN:0-7514-0158-7.

A: Gas Chromatography - kniha, I.A. Fowlis; ACOL, Wiley, 1995, ISBN:0-471-95467-5.

A: High Performance Liquid Chromatography - kniha, S. Lindsay; ACOL, Wiley, 1992, ISBN:0-471-93180-2.

A: Separace látek - skripta, M. Popl, J. Kubát; SNTL, 1986.

A: Chromatography Today - kniha, C.F. Poole, S.K. Poole; Elsevier, 1991, ISBN:0-444-88492-0.

Last update: Pátková Vlasta (05.01.2018)
Learning resources -

Last update: Pátková Vlasta (05.01.2018)
Teaching methods -

Every week there is a two hour lecture and one hour seminar.

Last update: Řezanka Pavel (31.01.2018)
Requirements to the exam -

1. The examination is written and takes 60 min.

2. Students, who study in Czech, pass examination in Czech. Students, who study in English, pass Examination in English.

3. Student is duty to present Index with written credit during the examination, otherwise it is impossible to continue in Examination.

4. The students sign in for the examination using SIS and it is duty of student to sign in for the term listed here. Signing off is possible up to date listed in SIS. Later it is possible only by request to secretary of department and only because of serious reasons. The examination is classified "F" when the student is absent and not signed off.

5. The assistant can allow writing examination when student arrives late. The missed time is not compensated.

6. It is not allowed to leave room during examination. It is not allowed to use forbidden materials and tools including telecommunication tools.

7. The place and the time will be announced during the examination. The examiner assign where and when will be possible to see checked examination test. If the student is not present in this time he or she resigns for any complain and agree with results. When student do not agree with classification of any of parts of test, the examiner will provide one, exceptionally more, additional question which will be answered in written form. According to result of these additional questions the examiner can change overall classification of examination by one step up or down.

8. Student can use only two reparative terms.

Last update: Řezanka Pavel (31.01.2018)
Syllabus -

1. Gas chromatograophy - detailed theory, van Deemter plots.

2. Gas chromatograophy - current trends.

3. High-performance chromatography - detailed theory, kinetic plots.

4. High-performance chromatography - current trends

5. Ultrahigh-performance chromatography.

6. Electrophoresis.

7. Capillary electrophoresis.

8. Separation on chips and high-throughput techniques.

9. Solid phase extraction - current status.

10. Simulated moving bead technology.

11. Supercritical fluid chromatography.

12. Mass spectrometry - 1.

13. Mass spectrometry - 2.

14. Webinars - useful source of information.

Last update: Sýkora David (19.01.2018)
Entry requirements -

The students need to know analytical chemistry on the level of Analytical chemistry I.

Last update: Pátková Vlasta (05.01.2018)
Registration requirements -

The students need to know analytical chemistry on the level of Analytical chemistry I.

Last update: Pátková Vlasta (05.01.2018)
Course completion requirements - Czech

Předmět je zakončen písemnou a ústní zkouškou. Pro udělení zápočtu student musí student ze zápočtového testu získat alespoň 50% bodů.

Last update: Řezanka Pavel (31.01.2018)
Teaching methods
Activity Credits Hours
Konzultace s vyučujícími 0.5 14
Účast na přednáškách 1 28
Příprava na přednášky, semináře, laboratoře, exkurzi nebo praxi 1 28
Příprava na zkoušku a její absolvování 1 28
Účast na seminářích 0.5 14
4 / 4 112 / 112
Coursework assessment
Form Significance
Examination test 60
Continuous assessment of study performance and course -credit tests 20
Oral examination 20